Many fungi growing on plant biomass produce proteins currently classified as glycoside hydrolase family 61 (GH61), some of which are known to act synergistically with cellulases. In this study we show that PcGH61D, the gene product of an open reading frame in the genome of Phanerochaete chrysosporium, is an enzyme that cleaves cellulose using a metal-dependent oxidative mechanism that leads to ...

Horseradish peroxidase was encapsulated in calcium alginate for the purpose of phenol removal. Upon immobilization, pH profile of enzyme activity changes as it shows higher value at basic and acidic solution. Investigation into time course of phenol removal for both encapsulated and free enzyme showed that encapsulated enzyme had nearly similar efficiency in comparison with the same concentrati...

The potential of enzymes recycling after hydrolysis and fermentation of wheat straw under a variety of conditions was investigated, monitoring the activity of the enzymes in the solid and liquid fractions, using low molecular weight substrates. A significant amount of active enzymes could be recovered by recycling the liquid phase. In the early stage of the process, enzyme adsorb to the substra...

D-amino acid oxidase from Trigonopsis variabilis (TvDAAO) was overproduced in Escherichia coli cells, and properties of the recombinant enzyme were studied. Single point mutants of the enzyme with 2.4-fold higher thermal stability or changed spectra of substrate specificity compared with wild-type enzyme were prepared. It was shown that mutant TvDAAO has higher catalytic efficiency in cephalosp...

The spatial organization of enzymes often plays a crucial role in the functionality and efficiency of enzymatic pathways. To fully understand the design and operation of enzymatic pathways, it is therefore crucial to understand how the relative arrangement of enzymes affects pathway function. Here we investigate the effect of enzyme localization on the flux of a minimal two-enzyme pathway withi...

Periodic enzyme activities can improve the metabolic performance of cells. As an adaptation to periodic environments or by driving metabolic cycles that can shift fluxes and rearrange metabolic processes in time to increase their efficiency. To study what benefits can ensue from rhythmic gene expression or posttranslational modification of enzymes, I propose a theory of optimal enzyme rhythms i...