Invertase is a commercially important enzyme used for the hydrolysis of sucrose. The hydrolysis of sucrose yields an equimolar mixture of glucose and fructose, known as invert syrup, is widely used in food and beverage industries. This enzyme is also used for the manufacture of artificial honey, plasticizing agents used in cosmetics, pharmaceutical and paper industries as well as enzyme electro...

Hydrogenases are attractive biocatalysts for utilization in electrochemical devices as potential replacement Pt hydrogen evolving electrodes. In this work, we investigate the immobilization of ferredoxin tagged FeFe-hydrogenase (Fd-HydA1) on black TiO2 nanotubes (bTNTs), with uniform nanotube opening diameters 140 nm. By utilizing an immunogold labelling method, show that enzymes attach top sur...

Poly(amidoamine) dendrimers having various degrees of modification with the redox-active ferrocenyls were prepared by controlling the molar ratio of ferrocenecarboxaldehyde to amine groups of dendrimers. By alternate layer-by-layer depositions of partial ferrocenyl-tethered dendrimers (Fc-D) with periodate-oxidized glucose oxidase (GOx) on a Au surface, an electrochemically and enzymatically ac...

Glucose oxidase catalyzes the formation of metallic gold particles in immediate proximity of the protein from gold (III) chloride in the absence of any other catalytic or reductive substrates. The protein-mediated gold reduction reaction leads to size-controllable gold particle formation and concomitant association of the enzyme in an electrically conductive metallic template. Such an enzyme im...

This paper describes a biomaterial microfabrication approach for interfacing functional biomolecules (enzymes) with electrode arrays. Poly (ethylene glycol) (PEG) hydrogel photopatterning was employed to integrate gold electrode arrays with the enzymes glucose oxidase (GOX) and lactate oxidase (LOX). In this process, PEG diacrylate (DA)-based prepolymer containing enzyme molecules as well as re...

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A novel enzyme electrode system for glucose oxidation was constructed by co-immobilization of dehydrogenase (GDH) and N,N’-diphenyl-p-phenylenediamine (DPPD) on carbon electrodes. This applied to porous fabric produce a current 7 mA/cm2, while the catalytic activity maintained at approximately 100% more than 4 h, indicating that DPPD molecules were stably retained electrodes during continuous r...

We report the usage of DNA hydrogels for enzyme entrapment in an enzymatic biobattery. With the recent advancements in DNA nanotechnology, the incorporation of DNA materials to bioelectrocatalytic electrodes holds great promise to improve the performance of bioelectrocatalysis-based devices.

The glucose oxidase (GOx) enzyme was immobilized on chitosan-based porous composite membranes using a covalent bond between GOx and the chitosan membrane. The chitosan-based porous membranes were prepared by the combination of the evaporation- and non-solvent-induced phase separation methods. To increase the membrane conductivity, carbon nanotubes (CNTs) were added to the chitosan solution. The...