( Asymmetric cellulose acetate membranes have been prepared and characterized in the laboratory with a view to use them in membrane bio-reactors. Enzymatic hydrolysis of sucrose has been investigated in a dead-end stirred cell type membrane bio-reactor using these membranes. Effect of different operating variables such as substrate concentration, enzyme loading, flow rate and working volume hav...

(C5H7N2)3(AsMo12O40)·2C5H6N2·1.5H2O was hydrothermally synthesized and structurally characterized by single-crystal X-ray diffraction. Crystal data: monoclinic, P21/n, a = 13.310(3), b = 22.399(5), c = 19.820(4) Å, β = 99.99(3) ◦, V = 5819(2) Å3, Z = 1, wR(F2) = 0.1345. The structure consists of Keggin-type [AsMo12O40]3− polyoxoanions. The title compound has a high catalytic activity for the ox...

Translation of redox biocatalysis into a commercial hydrogenation flow reactor, with in-built electrolytic H 2 generation, was achieved using immobilized enzyme systems. Carbon-supported biocatalysts were first tested in batch mode, and then transferred continuous columns for -driven, NADH-dependent asymmetric ketone reductions. The thus handled comparably to heterogeneous metal catalysts, but ...

Visualizing and measuring the gas distribution in close proximity to a working catalyst is crucial for understanding how the catalytic activity depends on the structure of the catalyst. However, existing methods are not able to fully determine the gas distribution during a catalytic process. Here we report on how the distribution of a gas during a catalytic reaction can be imaged in situ with h...

A novel implementation of in situ protein digestion supported by a graphene oxide-immobilized enzyme reactor (GO-IMER) in the MALDI imaging mass spectrometry (IMS) workflow is reported, which enables the simultaneous diagnostic identity and distribution attributes of the proteome on tissue.

A study was initiated to construct a micro-reactor for protein digestion based on trypsin-coated fused-silica capillaries. Initially, surface plasmon resonance was used both for optimization of the surface chemistry applied in the preparation and for monitoring the amount of enzyme that was immobilized. The highest amount of trypsin was immobilized on dextran-coated SPR surfaces which allowed t...