Escherichia coli O157 is the major cause of diarrhea-associated hemolytic uremic syndrome (HUS). Strains causing HUS contain either Shiga toxin 1 (Stx1) or Stx2, or both. In adult volunteers, conjugate vaccines of detoxified lipopolysaccharide (LPS) elicited bactericidal antibodies to E. coli O157. Here, the detoxified LPS was conjugated with improved schemes to the nontoxic B subunit of Stx1. ...

The value of a latex agglutination test (Escherichia coli O157 latex test; Oxoid Ltd.) for rapid presumptive detection of E. coli serotype O157:H7 was determined by laboratory trials and during an outbreak of hemorrhagic colitis. The latex test was found to be a simple, highly efficient and reliable test in detecting E. coli O157:H7 with 100% sensitivity and specificity. It was also found that ...

Escherichia coli O157:H7 (EC O157) is an important zoonosis. White-tailed deer (Odocoileus virginianus) have been implicated in transmission of this bacterium to humans and have been suggested as reservoirs that might affect carriage in cattle populations. Our study objectives were to estimate prevalence of EC O157 in feces of hunter-harvested deer and to describe fecal shedding patterns in a c...

Although cattle develop humoral immune responses to Shiga-toxigenic (Stx+) Escherichia coli O157:H7, infections often result in long-term shedding of these human pathogenic bacteria. The objective of this study was to compare humoral and cellular immune responses to Stx+ and Stx- E. coli O157:H7. Three groups of calves were inoculated intrarumenally, twice in a 3-week interval, with different s...

background and objectives: dairy cattle have been implicated as principal reservoir of verotoxin-producing escherichia coli (vtec), with undercooked ground beef and raw milk being the major vehicles of food borne outbreaks. vtec has been implicated as an etiological agent of individual cases and outbreaks in developed countries. this study was designed to determine the prevalence of vetec in di...

Escherichia coli O157:H7 and Non-O157 shiga toxin producing E. coli (STEC) serogroups O26, O45, O103, O111, O121, or O145 are both considered adulterated in non-intact beefs. Forty to fifty-eight percent of U.S. consumers prefer to order beef steaks of medium rare to rare status. From 2000 to 2007, undercooked non-intact beef products have been involved in several outbreaks in the United States...

BACKGROUND Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. METHODS We developed fluorescent amplification-based specific hybridization (FLASH-PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157:H7. RESULT PCR product of 336 bp was...

Two groups of calves were subjected to dietary stress by withholding of food beginning 1 or 14 days after inoculation with 10(10) CFU of Escherichia coli O157:H7. Following treatment, neither group had a significant increase in fecal shedding of E. coli O157:H7. A third group of calves had food withheld for 48 h prior to inoculation with 10(7) CFU of E. coli O157:H7. These calves were more susc...

background: enterohemorrhagic escherichia coli (ehec) o157:h7 is an infectious zoonotic pathogen causing human infections. these infections, in some cases, can lead to hemolytic uremic syndrome and its life-threatening complications and even death worldwide. the first intimate bacterial adhesion, intimin (i), with its own receptor translocated intimin receptor (tir) and e. coli secreted protein...