The rhizosphere is the area around the root of a plant occupied by a unique population of usefulbacteria known as plant growth promoting rhizobacteria (PGPR). In this study, the isolation andidentification of rhizobacteria from orange (Citrus sinensis) orchards using 16S rRNA gene, as well asbiological and biochemical assays is reported. Analysis of 16S rRNA gene was confirmed bybiological and ...

Proline dehydrogenase (ProDH; 1.5.99.8) belongs to superfamily of amino acid dehydrogenase, which plays a significant role in the metabolic pathway from proline to glutamate. The goal of this research was gene cloning and characterization of ProDH enzyme from Pseudomonas fluorescens pf-5 strain. The gene encoding ProDH was isolated by means of PCR amplification and cloned in an IPTG inducible T...

background and objectives: klebsiella pneumoniae ( k. pneumoniae ) is an opportunistic microorganism. this study aimed to investigate the presence of mag a gene and antimicrobial susceptibility in k. pneumoniae . materials and methods: 195 clinical specimens were collected from hospitals of shahrekord, iran. bacterial culture, biochemical diagnostic standard test, determination of antibiotic se...

proline dehydrogenase (prodh; 1.5.99.8) belongs to superfamily of amino acid dehydrogenase, which plays a significant role in the metabolic pathway from proline to glutamate. the goal of this research was gene cloning and characterization of prodh enzyme from pseudomonas fluorescens pf-5 strain. the gene encoding prodh was isolated by means of pcr amplification and cloned in an iptg inducible t...

background: asthma is very common in children and its diagnosis is based on clinical manifestations, which can be misdiagnosed as other respiratory diseases with similar signs and symptoms. objective: to analyze the expression of st2l and cd203c in the diagnosis of pediatric asthma. methods: basophils were purified from whole blood samples of patients and healthy controls using ficol-paque grad...

background: salmonella enterica serovar typhi (s. typhi) the cause of the acute febrile disease typhoid fever is the major public health problem in developing countries. asymptomatic carriers are the main sources of typhoid. the aim of this study was to investigate methods for isolation and identification of s. typhi in asymptomatic carriers. materials and methods: two hundred stool samples wer...

Background and Objective: The healthy people’s fecal flora in the community represents a large potential reservoir. Therefore, the current study aimed to detect antibiotic resistance patterns and virulence factors in Klebsiella pneumoniae isolated from healthy volunteers’ feces. Materials and Methods: Three hundred and fifty stool specimens were collected from sales rep healthy individuals ref...

Evaluation of gene expression in urinary sediment has been considered as a promising non-invasive approach for biomarker identification of kidney diseases. Nonetheless, there are several challenges in extraction of RNA from this valuable source of biomarkers, mostly because of the factors that have influence on quality of isolated RNA such as low cellular content. Accordingly, we compared the q...