Background Methods for haplotyping and DNA copynumber typing of single cells are paramount for studying genomic heterogeneity and enabling genetic diagnosis. Before analyzing the DNA of a single cell by microarray or next-generation sequencing, a whole-genome amplification (WGA) process is required, but it substantially distorts the frequency and composition of the cell’s alleles. As a conseque...

BACKGROUND Few studies comparing multiple methods for DNA extraction from dried blood spots (DBS) on filter paper for PCR targeting the Plasmodium genome have been done. METHODS Frequently-used methods for DNA extraction from DBS using Chelex-100, InstaGene Matrix, QIAamp DNA Mini Kit and TE buffer were compared on a dilution series of a standardized Plasmodium falciparum positive sample. The...

background: among the most important parasitic disease, causing diarrhea, gi­ardia lamblia is noteworthy. nowadays detection methods for these parasites in­clude parasitological methods such as microscopic examination. the sensitivity of these methods relies on the expertise and experience of examiners. in contrast, molecular methods such as pcr are less dependent on the expertise of the exam­i...

aim : the aim of this survey was to compare four dna extraction methods from iranian sheep strain e.granulosus isolates. background : cystic echinococcosis (ce) caused by the metacestode of the dog tapeworm echinococcus spp., is a global zoonotic infection which is economically important and constitutes a major threat to public health in many countries. strains characterization is essential for...

Background Methods for haplotyping and DNA copynumber typing of single cells are paramount for studying genomic heterogeneity and enabling genetic diagnosis. Before analyzing the DNA of a single cell by microarray or next-generation sequencing, a whole-genome amplification (WGA) process is required, but it substantially distorts the frequency and composition of the cell’s alleles. As a conseque...

Chrysanthemum morifolium is one of the most popular ornamental plants in world. However, as C. a segmental hexaploid, self-incompatible, and has sizable heterologous genome, it difficult to modify its trait systematically. Genome editing technology attractive methods for modifying traits For commercial use genetically modified morifolium, rigorous stabilization quality essential. This stability...

background: dna immunization with plasmid dna encoding bacterial, viral, parasitic, and tumor antigens has been reported to trigger protective immunity. the use of plasmid dna vaccinations against many diseases has produced promising results in animal and human clinical trials; however, safety concerns about the use of dna vaccines exist, such as the possibility of integration into the host gen...

Methylated and un-methylated control DNA is an important part of DNA methylation studies. Although human and mouse DNA methylation control sets are commercially available, in case of methylation studies on other species such as animals, plants, and bacteria, control sets need to be prepared. In this paper a simple method of generating methylated and un-methylated control DNA is described. Whole...