نتایج جستجو برای: glycoproteins
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The influenza virus uses the hemagglutinin (HA) and neuraminidase (NA) glycoproteins to interact with and infect host cells. While biochemical and microscopic methods allow examination of the early steps in flu infection, the genesis of progeny virions has been more difficult to follow, mainly because of difficulties inherent in fluorescent labeling of flu proteins in a manner compatible with l...
A simple and efficient method for selectively imaging and monitoring in situ the expression of sialylated glycoproteins on living cells has been developed. Treating living cells by mild periodate oxidation to selectively generate aldehydes on sialylated glycoproteins, followed by direct labeling of aldehydes with a commercially available fluorescent tag, fluorescein-5-thiosemicarbazide (FTSC), ...
Graphical Abstract The cover pictures shows examples of bioorthogonal chemistry and glycoscience integration made possible through methods pioneered by Carolyn Bertozzi. Metabolic oligosaccharide engineering enables the detection glycan structures in living systems, while facile construction glycoconjugates allows for installation defined glycoproteins cutting-edge antibody tools.
The glycoproteins HN and F and the lipids were solubilized from Sendai virus envelopes by using the nonionic detergent beta-D-octylglucoside. When beta-D-octylglucoside was removed by dialysis, the glycoproteins and lipids reassociated to form vesicles. These vesicles displayed hemagglutinating, neuraminidase, and hemolysin activities comparable to those expressed by the intact virus. The vesic...
S-glycoproteins, i.e. stigma glycoproteins that are heritable in correlation with S allele in self-incompatible Brassica species, were apparently purified for three S alleles in B. oleracea. From SDS gel electrophoresis, the estimated molecular weight for two of the S-glycoproteins was 57,000. The other S-glycoprotein was considered to be heterogeneous with molecular weights of 60,000 and 65,00...
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