SOURCE/DESCRIPTION: pB12, a 650 bp cDNA cloned at the Pst I and Hind III sites of pSP64 (Spindel et. al.). P0LYM0RPHIS: Pvu II detects two allelic fragments at 8.9 kb and 8.3 kb. OTHER COMMENTS: To obtain better separation of bands electrophorese on a 0.8% agarose gel until the xylene cyanol dye is at least 9 cm from the origin. ACKNOWLEDGEMENTS: We thank Ms. Denise Boorman for technical help.

Hydropericardium syndrome (HPS) agent was purified by sucrose gradient centrifugation. The polypeptides of HPS agent were separated through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and identified by known protein markers. Eight polypeptides having molecular weights ranging from 119.0 to 15.7 KDa were observed. The nucleic acid of HPS agent was extracted and determin...

In Synechocystis 6803 the psbA gene, encoding the Dl polypeptide of the Photosystem II reaction center, belongs to a multigene family with three copies (1). The nucleotide sequence of psbA-1 has been reported (2). We have cloned and seguenced a seconcl psbA gene, psbA-2. front Svnechocvstis 6803. The second copy of psbA i s contained within a 2.1 Kb genomic Hind III fragment. The psbA-2 open re...

I. Experiments in which the spinal cord is cut 24 II. Experiments in which the peripheral reflexes are tested during narcosis progression ..27 III. Movements of narcosis progression after de-afferentation of one hind limb . .32 IV. The general bearing of these experiments . . 33 V. The fundamental conditions of rhythmic progression 34 VI. The nature and position of the reflex . . . 39 VII. Evol...

objective(s): this study aims to investigate joint association between cholesterol ester transfer protein (cetp) polymorphisms and body mass index (bmi) or birth weight with the risk of dyslipidemia in iranian children and adolescents. materials and methods:this study was conducted as a sub-study of the “school-based nationwide health survey” (caspian-iii). we randomly selected 750 samples from...

purpose: microgravity causes major changes in various systems of the body in space, such as shift in cerebrospinal fluid, decreased red blood cells count, and electrolyte loss. these complications are very important in space and we should find new approaches to prevent the side effects of microgravity in astronauts. materials and methods: this experimental study was conducted on 21 adult male r...

Purpose: Microgravity causes major changes in various systems of the body in space, such as shift in cerebrospinal fluid, decreased red blood cells count, and electrolyte loss. These complications are very important in space and we should find new approaches to prevent the side effects of microgravity in astronauts. Materials and Methods: This experimental study was conducted on 21 adult ma...

With the binary vectors pBIG and pBIB it is possible to: i) exchange the plant selectable marker/promoter fusion and the reporter gene, ii) use one vector and Agrobacterium strain system, thus reducing variables associated with using different vectors and strains, iii) allow for divergent transcription of the selectable marker gene and of promoter/reporter gene fusions. The two plasmids are der...

The live weight of bulls at 9 months age groups II, III and IV (crossbreed 1) was 305.6, 304.1 307.6 kg, respectively, compared with peers group I 15.3 kg (5.0%), 13.8 (4.5%) 17.3 (5.6%) were higher (P≤0.001). Also, for a 9-month growth period, the average daily gain in II 1018.5 g, which amounted to 62.2 g or 6.1% (P≤0.001), 25.9 2.5% (P≤0.01) 24.4 2.4%. (P≤0.01). According external parameters...