Combined QM/MM calculations of an activesite of Cytochrome P450cam have been performed, before and after a binding process of Cytochrome P450cam to Putidaredoxin. The calculations have been carried out for two coordination spheres of the heme active-site of Cytochrome P450cam, namely a 5-coordinated, and a 6coordinated system of this protein having a water molecule as a 6th ligand. An experimen...

The formation of neutral I2 by the photodissociation of the methyl iodide dimer, (CH3I)2, excited within the A band at 249.5 nm is evaluated using velocity map imaging. In previous work [J. Chem. Phys. 122, 204301 (2005)], we showed that the formation of I2+ from photodissociation of the methyl iodide dimer takes place via ionic channels (through the formation of (CH3I)2+). It is thus not possi...

Previous studies have demonstrated that mutations of highly conserved residues in the alpha subunit of Gs (alpha s) can inhibit either the intrinsic GTPase activity (glutamine-227 to leucine, Q227L) or the ability of the protein to be activated by GTP (glycine-226 to alanine, G226A). We stably transfected NIH 3T3 cells with cDNAs encoding Gi2 alpha subunit (alpha i2) containing either wild-type...

Background Optical coherence tomography (OCT) is one of the most valuable imaging techniques in evaluation macula. The repeatability and reproducibility OCT are crucial importance reassuring this tool’s efficacy evaluating structure retina. Objective This study aims to investigate measuring macular thickness normal eyes before after pupil dilation. Methods A total 44 healthy individuals age ran...

PURPOSE We evaluated the day-to-day variability of transcutaneous oxygen tension (tcPO2) in patients with diabetes mellitus and peripheral arterial occlusive disease who were at risk for chronic foot ulceration. METHODS The tcPO2 was measured in the morning once daily for 3 consecutive days in 10 male patients with diabetes mellitus who were hospitalized. The mean age of the patients was 65 +...