This work describes the synthesis and use of the chelator lipid, nitrilotriacetic acid ditetradecylamine (NTA-DTDA). This lipid is readily dispersed in aqueous media, both alone and when mixed with carrier lipids like dimyristoylphosphatidylcholine (DMPC). Fluorescence microscopic examination of membranes deposited from NTA-DTDA-containing liposomes shows that NTA-DTDA mixes uniformly with the ...

Protein-protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli ORF clone library. Of 4339 bait proteins tested...

FhuA (Mr 78,992, 714 amino acids), siderophore receptor for ferrichrome-iron in the outer membrane of Escherichia coli, was affinity tagged, rapidly purified, and crystallized. To obtain FhuA in quantities sufficient for crystallization, a hexahistidine tag was genetically inserted into the fhuA gene after amino acid 405, which resides in a known surface-exposed loop. Recombinant FhuA405.H6 was...

Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an autoantigen in patients with certain autoimmune diseases. The individual subunits of Ku have been difficult to isolate from human cells without denaturation and attempts to produce functional recomb...

The alpha1- and beta1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purification of active recombinant enzyme on a nickel-affinity column. The enzyme has one heme p...

Title of Document: LONGITUDINAL SPACE-CHARGE WAVES INDUCED BY ENERGY MODULATIONS Brian L. Beaudoin, Master of Science, 2008 Directed By: Professor Patrick G. O’Shea and Professor Rami A. Kishek Department of Electrical and Computer Engineering High intensity beams that are space-charge dominated are important for applications such as free electron lasers that require high intensity and low emit...

Electronic Orientation Aids (EOA) usually guide visually impaired pedestrians using turn-by-turn instructions. However, several studies have demonstrated that mental representations of space seem difficult to build when turn-by-turn instructions only are provided. This is a crucial point as getting an accurate spatial representation of the surroundings can make the difference between successful...

A fast photodiode was demonstrated to be able to extract the turn by turn photon beam profile information of electron storage rings if the photodiode was operated at the saturated region. When applied this method to a 1.3 GeV synchrotron light source with 200 mA beam current, the synchrotron radiation intensity is not intense enough to saturate the photodiode. To apply this method to the type o...

Copy Summary: It has been found that humans not only tend to avoid the middle routes and prefer the peripheral routes among multiple choices, but also rely on the ‘initial segment strategy’ to select the route. In this paper, we propose a new heuristic which humans apply during route selection: Participants prefer the route whose initial direction lies in the direction of their final destinatio...