Background This novel approach describes a rapid and simple method for identification of necrotic vs. viable cells within a mammalian blastocyst. MaterialsAndMethods Hatched bovine blastocysts produced in vitro were first incubated for 30 min in pre-equilibrated culture medium containing propidium iodide (PI; 300μg/ml) and bisbenzimide (Hoechst: H33342; 5μg/ml) fluorescent dyes. Embryos were th...

Blinatumomab, the bispecific T cell engager, has been demonstrated as the most successful BsAb to date. Throughout the past decade, vector design has great importance for the expression of monoclonal antibody in Chinese hamster ovary (CHO) cells. It has been indicated that expression plasmids based on the elongation factor-1 alpha (EF-1 alpha) gene and DHFR selection marker can be highly effect...

To explore the function of kaempferol on lung cancer cell A549 and microRNA-199 variation is objective study. The proliferation was detected by counting kit-8. expression mammalian target rapamycin, phosphorylated light chain 3-?/?, beclin-1, B-cell lymphoma 2 2-associated X protein showed Western blot. determined quantitative polymerase reaction. adenocarcinomic human alveolar basal epithelial...