BACKGROUND Manufacture of MATa and MATα yeast cells is required for crossbreeding, a procedure that permits hybridization and the generation of new heterozygous strains. Crossbreeding also can be performed with a- and α-type of cells, which have the same mating abilities as MATa and MATα haploid cells, respectively. RESULTS In this work, we describe a method to generate a- and α-type of cells...

Article history: Received 21 September 2009 Received in revised form 23 August 2010 Accepted 23 August 2010 Available online 8 September 2010 Recent years have witnessed an increased interest in computing cosine similarity in many application domains. Most previous studies require the specification of a minimum similarity threshold to perform the cosine similarity computation. However, it is us...

Previous studies demonstrated that mitochondrial DNA (mtDNA) was uniparentally transmitted in laboratory crosses of the pathogenic yeast Cryptococcus neoformans. To begin understanding the mechanisms, this study examined the potential role of the mating-type locus on mtDNA inheritance in C. neoformans. Using existing isogenic strains (JEC20 and JEC21) that differed only at the mating-type locus...