Tryptanthrin, a kind of indole quinazoline alkaloid, has been shown to exhibit anti-microbial, anti-inflammation and anti-tumor effects both in vivo and in vitro. However, its biological activity on human chronic myeloid leukemia cell line K562 is not fully understood. In the present study, we investigated the proliferation-attenuating and apoptosis-inducing effects of tryptanthrin on leukemia ...

The issue of context arises in various areas of AI, including knowledge representation, natural language processing, and intelligent information retrieval. Although the word context is frequently used in descriptions, explanations, and analyses of computer programs in these areas, its meaning is frequently left to the reader’s understanding; that is, it is used in an implicit and intuitive mann...

< a d d a r t t y p e = " r e l " d o i = " 1 0. 1 0 8 3 / j c b. 2 0 1 5 0 8 0 9 9 " > Otera et al.< / a d d a r t > reveal that the mito-chondrial fi ssion factor Drp1 and its receptors MiD49 and MiD51 promote apoptosis by remodeling mitochondrial cristae. Early in apoptosis, mitochon-drial cristae are remodeled so that cytochrome c enters the space between the inner and outer mito-chondrial ...

prosthetic joint operations in Japan: a 12-month cohort study in a teaching hospital Tomoko Sakihama, RN, CNSICN, MSN; Mikiro Kato, MD; Ryoko Harada, RN, CNA; Yasuharu Tokuda, MD MPH; Department of Nursing International University of Health and Welfare Graduate School, Tokyo, Japan; Division of Medicine Mito Kyodo General Hospital University of Tsukuba, Ibaraki, Japan; Department of Nursing, Mi...

Most apoptotic stimuli require mitochondrial outer membrane permeabilization (MOMP) in order to execute cell death. As such, MOMP is subject to tight control by Bcl-2 family proteins. We have developed a powerful new technique to investigate Bcl-2-mediated regulation of MOMP. This method, called mito-priming, uses co-expression of pro- and anti-apoptotic Bcl-2 proteins to engineer Bcl-2 addicti...

We mentioned in the introduction to Chapter 5 that MYCIN provided a starting point for several additional research projects. The Ventilator Manager (VM) project of Larry Fagan had its beginnings in the MYCIN project but quickly diverged because of the dynamic nature of the intensive care unit (ICU) setting for which it was designed. MYCIN required a "snapshot" approach to patient assessment-tem...

As AIM researchers began to develop techniques for allowing systems to explain their reasoning, some researchers became intrigued by the potential educational role of the developing methods. It became clear that advanced computer-aided instruction (CAl) programming techniques could be applied and extended in the medical setting. Intelligent computer-aided instruction (ICAI) differs from traditi...

Regulation of cytosolic Ca(2+) concentration ([Ca(2+)](cyt)) in airway smooth muscle (ASM) is a key aspect of airway contractility and can be modulated by inflammation. Mitochondria have tremendous potential for buffering [Ca(2+)](cyt), helping prevent Ca(2+) overload, and modulating other intracellular events. Here, compartmentalization of mitochondria to different cellular regions may subserv...

Mitochondria generate most of the heat in endotherms. Given some impedance of heat transfer across protein-rich bioenergetic membranes, mitochondria must operate at a higher temperature than body temperature in mammals and birds. But exactly how much hotter has been controversial, with physical calculations suggesting that maximal heat gradients across cells could not be greater than 10-5 K. Us...

Pepe, Salvatore, Naotaka Tsuchiya, Edward G. Lakatta, and Richard G. Hansford. PUFA and aging modulate cardiac mitochondrial membrane lipid composition and Ca21 activation of PDH. Am. J. Physiol. 276 (Heart Circ. Physiol. 45): H149–H158, 1999.—Aberrations in cell Ca21 homeostasis have been known to parallel both changes in membrane lipid composition and aging. Previous work has shown that the l...