background: cryptosporidium spp. is a coccidian parasite infected humans and animals. prevalence rate of cryptosporidium spp. infection associated with is some parameters such as sampling, age, season, country and contact to domestic animals. this study aimed to determine cryptosporidium spp. infection in humans and some animals in rural areas of shushtar district from khuzestan province, south...

background : this is the first work done on cryptosporidiosis among the children in taiz, yemen. methods : a number of 712 samples were collected from children of different ages (ranging from 1 month to 12 years) from dec 2006 to aug 2007. the collected samples were examined by sheather's sugar floatation and modified ziehl- neelsen stain as well as elisa methods. the test results were statisti...

We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in...

PURPOSE To investigate the contamination of the dental irrigation systems with Cryptosporidium species in Alexandria, Egypt. METHODS Forty water samples from all 20 working dental irrigation machines in a dental center in Alexandria were included in the study. Water samples were taken from the handpieces of dental irrigation machines in all studied units. After filtration through a membrane f...

The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stain...

The aim of this prospective study was to determine the prevalence of Cryptosporidium parvum in hospitalized children in Kota Bharu, Malaysia. Over a 19 month study period, 258 stool samples were examined from 159 children; 109 with diarrhea and 50 controls without diarrhea. Modified Ziehl-Neelsen staining method and a polymerase chain reaction (PCR) assay were used to detect C. parvum and the s...

A cross sectional study was carried out from November 2014 to April 2015 to determine the prevalence of Cryptosporidiosis in calves, lambs and goat kids and to assess potential risk factors in Bishoftu, of Oromia regional state, Ethiopia. Hence, fecal samples from a total of 364 study animals (214 calves, 89 lambs and 61 kids) were examined with Sheather’s flotation technique and Modified Ziehl...

neonatal calf diarrhea due to cryptosporidium is a major health problem. it causes extensive economic losses. this study was carried out on newborn calves in different parts of east azarbayjan province over november 2001 - 2002. five hundred feacal samples were taken from diarrheic calves under the age of 2 months from 25 commercial dairies and referral cases to the private clinics in four dist...

BACKGROUND Cases of cryptosporidiosis have not been officially reported in Estonia after the year 2000, and the disease appears to be either under-diagnosed or under-reported. FINDINGS Based on a human case of cryptosporidiosis contracted during faecal sampling in dairy farms, cattle considered to be sources of infection were analysed for Cryptosporidium spp. by a modified Ziehl Neelsen techn...

BACKGROUND The role of opportunistic infections in accelerating disease progression in HIV-positive individuals, leading to quick death, is still receiving serious attention. The objective of this study was to determine the prevalence of Isospora belli infections in HIV-positive patients in Edo State, Nigeria between August 2007 and March 2008. METHODS A total of 268 samples from HIV-positive...