The high attrition rate of in vitro human embryo culture presents a major obstacle in the treatment of clinical infertility by in vitro fertilization (IVF). Physical and genetic requirements are not well understood for human or mouse preimplantation embryo development. Group culture is an established requirement for optimal embryo development in the mouse model. However, conventional microdrop ...

The first report of replication of Riley's plasma enzyme-elevating virus (Riley virus) in tissue culture was by Yaffe (1), 2 years after the discovery of the virus in 1960 (2). Yaffe found that Riley virus multiplied in primary mouse embryo cultures until the death of the cultures through overcrowding (3 to 4 weeks), without producing cytopathic or other detectable changes, and could be seriall...

Criteria for assessing embryo vitality of denuded rabbit and mouse embryos in vitro were: volume of the embryo, number of cells and number of metaphase plates after 24 and 48 h in culture. Mouse embryos grew normally, with and without the zona pellucida, but denuded rabbit embryos showed reduced growth (volume and cell number) and failure to implant in synchronized recipients.

Abstract At the moment of union in fertilisation, sperm and oocyte are transcriptionally silent, but little is understood about how transcription initiates newly-formed embryo. In this talk, I shall suggest why fundamental process has remained arcane for so long, my lab sought to unlock its secrets mouse human embryos. By combining micromanipulation single-cell RNA-sequencing, we have revealed ...

Although the biokinetics, metabolism, and chemical toxicity of atorvastatin calcium are well known, until recently little attention was paid to the potential toxic effects of atorvastatin calcium on re-production and development in mammals. In recent years, it has been shown that atorvastatin calcium is a developmental toxicant when given orally or subcutaneously (SC) to mice. Decreased fertili...

Background: Embryo vitrification was effectively used for assisted reproductive techniques. Despite the undeniable benefits of vitrification, cooling and warming stress, and cytotoxicity of cryoprotectant may affect the DNA methylation that have an important role in gene activation and silencing. In the present study effects of 2-cell embryo vitrification on DNA methylation in hatched blastocys...

Background: In recent years, a combination of hormones and agents in assisted reproductive technology (ART) cycles as a means of stimulating the ovary and developing more follicles. With respect to the positive effects of Estradiol Valerate on reproductive tissues and the impact on native genes of the egg that transferred to the embryo after fertilization, it seems that this agent can also effe...

Background: The cryopreservation of human oocyte would make significant contribution to fertility treatments. It could also provide on alternative to embryo preservation for avoidance ethical problems. Embryo cryopreservation is now a successful procedure, but oocyte cryopreservation has poorer results. Materials and Methods: For retrieval of oocyte NMRI mouse were used. after administration of...