DNA methylation contributes to carcinogenesis by silencing key tumor suppressor genes. Here we report an ultrasensitive and reliable nanotechnology assay, MS-qFRET, for detection and quantification of DNA methylation. Bisulfite-modified DNA is subjected to PCR amplification with primers that would differentiate between methylated and unmethylated DNA. Quantum dots are then used to capture PCR a...

Diagnosis of respiratory viruses traditionally relies on culture or antigen detection. We aimed to demonstrate capacity of the reverse transcription polymerase chain reaction/electrospray ionization mass spectrometry (RT-PCR/ESI-MS) platform to identify clinical relevant respiratory viruses in nasopharyngeal aspirate (NPA) samples and compare the diagnostic performance characteristics relative ...

The new year is a time for goal setting, reflection, and new beginnings. Often, resolutions begin with self-improvements of the physical kind-losing weight, exercising, eating clean. These are important ideas and goals, although often difficult to sustain in part because sustaining is an implied element of each. Intentions are different than goals in the sense that an intention can be implement...

The aim of this study was to investigate the microRNA (miRNA) signature in atrial fibrillation (AF) with mitral stenosis (MS). miRNA arrays were used to evaluate the expression signature of the right atrial appendages of healthy individuals (n=9), patients with MS and AF (n=9) and patients with MS without AF (n=4). The results were validated with qRT-PCR analysis. GOmir was used to predict the ...

In this article, we show that high resolution melting analysis (HRM) is a sensitive and specific method for the detection of methylation. Methylated DNA and unmethylated DNA acquire different sequences after bisulphite treatment resulting in PCR products with markedly different melting profiles. We used PCR to amplify both methylated and unmethylated sequences and assessed HRM for the determina...

In this article, we show that high resolution melting analysis (HRM) is a sensitive and specific method for the detection of methylation. Methylated DNA and unmethylated DNA acquire different sequences after bisulphite treatment resulting in PCR products with markedly different melting profiles. We used PCR to amplify both methylated and unmethylated sequences and assessed HRM for the determina...

Francisella tularensis is the cause of the zoonotic disease tularemia and is classifi ed among highly pathogenic bacteria (HPB) due to its low infection dose and potential for airborne transmission. In the case of HBP, there is a pressing need for rapid, accurate and reliable identifi cation. Phenotypic identifi cation of Francisella species is inappropriate for clinical microbiology laboratori...

multiple sclerosis (ms) is a chronic inflammatory disease that leads to degeneration of the brain and spinal tissue. imbalances of cd4+ t cells including thelper1 (th1)/thelper2 (th2) and thelper17 (th17)/tregulatory (treg), their secreted cytokines and gene expressions, are important aspects of in immunopathogenesis of ms. vitamin a and its metabolites can regulate the immune system and appear...

The “in house” PCR method and four commercial PCR kits (designed symbolically as A, B, C, and D) for the detection of Mycoplasma synoviae (MS) in tracheal swabs of infected experimentally SPF chickens were compared. The chickens were inoculated intranasally with 1x10 colour change unit (cfu)/mL of ATCC reference MS strain, and at 4, 7, 14, 28, and 35 d post infection (d.p.i.), the tracheal samp...