نتایج جستجو برای: ms pcr

تعداد نتایج: 298173  

2016
Kristoffer Strålin Fredrik Ehn Christian G Giske Måns Ullberg Jonas Hedlund Johan Petersson Carl Spindler Volkan Özenci

We studied the diagnostic performance of the IRIDICA PCR/electrospray ionization-mass spectrometry (PCR/ESI-MS) assay applied on bronchoalveolar lavage (BAL) samples, from 51 mechanically ventilated patients with suspected pneumonia, in a prospective study. In 32 patients with X-ray verified pneumonia, PCR/ESI-MS was positive in 66% and BAL culture was positive in 38% (p = 0.045), and either of...

Journal: :Phytopathology 2008
E Postinikova C Baldwin C A Whitehouse A Sechler N W Schaad R Sampath V Harpin F Li R Melton L Blyn J Drader S Hofstadler W L Schneider

Polymerase chain reaction/electrospray ionization-mass spectrometry (PCR/ESI-MS, previously known as "TIGER") utilizes PCR with broad-range primers to amplify products from a wide array of organisms within a taxonomic group, followed by analysis of PCR amplicons using mass spectrometry. Computer analysis of precise masses allows for calculations of base compositions for the broad-range PCR prod...

2010
Chiara Azzari Maria Moriondo Giuseppe Indolfi Martina Cortimiglia Clementina Canessa Laura Becciolini Francesca Lippi Maurizio de Martino Massimo Resti

BACKGROUND Pneumococcal serotyping is usually performed by Quellung reaction, considered the gold standard test. However the method cannot be used on culture-negative samples. Molecular methods can be a useful alternative. The aim of the study was to evaluate the use of Multiplex-sequential-PCR (MS-PCR) or Realtime-PCR on blood samples for diagnosis and serotyping of invasive pneumococcal disea...

2010
Christel Steen Nadine Wilczak Johannes M. Hoogduin Marcus Koch Jacques De Keyser

BACKGROUND Two studies using (31)P-magnetic resonance spectroscopy (MRS) reported enhanced phosphocreatine (PCr) levels in normal appearing white matter (NAWM) of subjects with multiple sclerosis (MS), but this finding could not be properly explained. METHODOLOGY/PRINCIPAL FINDINGS We performed (31)P-MRS and (1)H-MRS in the NAWM in 36 subjects, including 17 with progressive MS, 9 with benign ...

Journal: :Clinical chemistry 2011
Erin J Kaleta Andrew E Clark Abdessalam Cherkaoui Vicki H Wysocki Elizabeth L Ingram Jacques Schrenzel Donna M Wolk

BACKGROUND Emerging technologies for rapid identification of microbes demonstrate a shift from traditional biochemical and molecular testing algorithms toward methods using mass spectrometry (MS) for the semiquantitative analysis of microbial proteins and genetic elements. This study was performed to assess the diagnostic accuracy of 2 such technologies, PCR-electrospray ionization (ESI)/MS and...

Journal: :Journal of clinical microbiology 2014
Yu-Tzu Chang Hsuan-Chen Wang Ming-Cheng Wang An-Bang Wu Junne-Ming Sung H Sunny Sun Ih-Jen Su Wei-Chih Kan Chih-Chiang Chien Jyh-Chang Hwang Hsien-Yi Wang Chin-Chung Tseng Chi-Jung Wu

PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of ...

2017
Måns Ullberg Petra Lüthje Paula Mölling Kristoffer Strålin Volkan Özenci

The clinical demand on rapid microbiological diagnostic is constantly increasing. PCR coupled to electrospray ionization-mass spectrometry, PCR/ESI-MS, offers detection and identification of over 750 bacteria and Candida species directly from clinical specimens within 6 hours. In this study, we investigated the clinical performance of the IRIDICA BAC LRT Assay for detection of bacterial pathoge...

A. Ashtari F. Elhamnia G.R. Shokri M. Banani S.A. Pourbakhsh,

Mycoplasma synoviae (MS) is an important avian pathogen that can cause both respiratory disease and joint inflammation synovitis in poultry, inducing economic losses to the Iranian chicken industry especially breeder farms. The aim of this study was to use the MS specific PCR and culture methods in order to detect of M. synoviae from breeder farms where located in Tehran province. A total of 47...

Journal: :Journal of clinical microbiology 2014
Andrea Bacconi Gregory S Richmond Michelle A Baroldi Thomas G Laffler Lawrence B Blyn Heather E Carolan Mark R Frinder Donna M Toleno David Metzgar Jose R Gutierrez Christian Massire Megan Rounds Natalie J Kennel Richard E Rothman Stephen Peterson Karen C Carroll Teresa Wakefield David J Ecker Rangarajan Sampath

The rapid identification of bacteria and fungi directly from the blood of patients with suspected bloodstream infections aids in diagnosis and guides treatment decisions. The development of an automated, rapid, and sensitive molecular technology capable of detecting the diverse agents of such infections at low titers has been challenging, due in part to the high background of genomic DNA in blo...

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