نتایج جستجو برای: multiplex real

تعداد نتایج: 548088  

Journal: :Respiration; international review of thoracic diseases 2014
Ryo Tachikawa Keisuke Tomii Ryutaro Seo Kazuma Nagata Kyoko Otsuka Atsushi Nakagawa Kojiro Otsuka Hisako Hashimoto Ken Watanabe Norio Shimizu

BACKGROUND Human herpes viruses (HHVs) are important pathogens in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). Rapid and efficient diagnostic tools are needed to detect HHVs in the lung in ALI/ARDS patients. OBJECTIVES This study aimed to evaluate the usefulness of multiplex and real-time polymerase chain reaction (PCR) analysis of bronchoalveolar lavage fluid (BALF...

Journal: :Phytopathology 2009
Y Ha A Fessehaie K S Ling W P Wechter A P Keinath R R Walcott

To improve the simultaneous detection of two pathogens in cucurbit seed, a combination of magnetic capture hybridization (MCH) and multiplex real-time polymerase chain reaction (PCR) was developed. Single-stranded DNA hybridization capture probes targeting DNA of Acidovorax avenae subsp. citrulli, causal agent of bacterial fruit blotch, and Didymella bryoniae, causal agent of gummy stem blight,...

2015
Manizheh Alami

Recently, studies on spoken language in real-life contexts increased dramatically. As a result, some of the features previously considered „empty‟, „superfluous‟ and redundantsuch as sort of, y‟know and well (Goddard and Meanpatterson, 2000, p. 98) now are considered as crucial aspects of interpersonal communication. These expressions called „discourse markers‟ (Schiffrin, 1987) or „pragmatic e...

Journal: :Ophthalmology 2021

PurposeCurrent polymerase chain reaction (PCR) methods for the diagnosis of infections are time consuming and require large sample volume skilled technicians. We developed a novel, easy-to-use, rapid (processing time, 1 minute; total 33 minutes) multiplex real-time PCR test (Direct Strip PCR) that did not DNA extraction to detect 9 pathogens could cause uveitis in 20-μl samples.DesignMulticente...

Journal: :European Journal of Clinical Microbiology & Infectious Diseases 2011

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