We have assayed the ability of various lipids to affect DNA polymerases activity in a DNA-membrane complex extracted from Streptococcus pneumoniae by the Sarkosyl-M-band technique. In addition, to determine which DNA polymerases were affected by the lipids, we partially purified three DNA polymerase activities from cell lysates, the first such demonstration outside of Escherichia coli and Bacil...

The protease inhibitive assay for heavy metals is a novel and robust detection method suitable for preliminary screening method for detecting heavy metals in agriculture and environment samples. Stem bromelain has been previously showed to be sensitive towards mercury and copper. However, commercial preparation is in crude form and contains possible interfering entities. Using ion exchange and ...

Peroxisomal matrix proteins were extracted from the highly purified peroxisomes with sodium pyrophosphate, and the membranes were sedimented by high speed centrifugation. Biochemical marker enzyme analyses revealed a quantitative release of a number of well-known peroxisomal matrix proteins from the purified peroxisomes. In contrast, carnitine medium/long chain acyltransferase activity, assayed...

In this report, the changes in the sedimentation character istics of the E. coli enzyme in the presence of nucleotides are confirmed. However, no alterations in the sedimentation constant were noted with the enzyme , which was partially purified from regenerating rat liver. The latter enzyme also exhibited a markedly different mobility in an electrophoretic field than E. coli TdR kinase. labora...

The (sodium -Ipotassium)-activated adenosine triphosphatase (NaK ATPase) in Lubrol extracts of NaI-treated bovine brain microsomes has been purified 30 to 50 times over that in the microsomes by salt and isoelectric precipitation, zonal centrifugation, and a new ammonium sulfate fractionation procedure. The final step in the purification procedure renders the NaK ATPase insoluble. The partially...

Two vesicular fractions and one nonvesicular fraction were prepared from crude synaptosomes by differential centrifugation and salting out with ammonium sulfate. Fraction 1 contained a mixture of coated vesicles, material thought to be derived from breakdown of the coats (shell fragments), and plain synaptic vesicles. Fraction 2 contained a mixture of plain synaptic vesicles and flocculent mate...

The protease from Chromohalobacter japonicus BK-AB18 was produced by growing bacteria in a LB medium containing 5% casein and NaCl. crude partially purified three levels of ammonium sulfate concentration (ranges 0–70%, 70–75% 75–80%) the highest specific activity exhibited range 75–80%. enzyme has relative molecular weight 65 kDa. this fraction had following optimum conditions: 7.5% NaCl, pH 9....

The demand for natural antioxidants has increased due to the harmful effects of synthetic antioxidants, such as toxicity and carcinogenic properties. Microalgae face this requirement they can produce numerous substances that have potential be antioxidants. This study aimed evaluate antioxidant activity Cosmarium extracts using ABTS radical cation assay identify compounds probably responsible it...

NADP-dependent glutamate dehydrogenase was partially purified from extracts of the marine siphonous green alga Caulerpa simpliciuscula. The enzyme had an apparent Km NH(4) (+) of 0.4 to 0.7 mm and was highly specific for NADPH, alpha-ketoglutarate, and ammonium ions.The bulk of the NADP-glutamate dehydrogenase was isolated with the chloroplast fraction in cell-free preparations of this alga and...