cacl2 treatment followed by heat shock is the most common method for artificial transformation. here, the cells were transformed using cacl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. the bl21 strain of escherichia coli (e. coli) was being susceptible using cacl2 treatment. some cells were ...

The inhomogeneous strain distribution introduced by the tensile deformation of pearlitic steel was visualized using digital image correlation method, and morphology cementite quantitatively evaluated. high near block boundaries. Within blocks, inhomogeneously distributed. ratio (Lcem/Lcol) length to colony size, which is related degree spheroidization, well correlated with average within coloni...

Corrosion performance on pearlite structures of carbon steels has been researched using scanning Kevin probe force microscopy and energy-dispersive X-ray spectroscopy. The corrosion test was conducted by immersing in borate buffer solution containing NaCl. In the coarser structure, we detected an increase Volta potential at pearlitic cementite–ferrite boundaries after corrosion, accordance with...