A few years ago it was found ls2 that the effect of pure gelatin and crystalline egg albumin on the conductivity of a 0.6 per cent NaCl solution is dependent on the hydrogen ion concentration. Additional increments of protein increased total conductivity when the proteins were highly ionized (pH 3.3) and decreased it when they were relatively less ionized (pH 5.1 and 7.4). At the reaction of hu...

Insulin is an important biotherapeutic protein, and it is also a model protein used to study amyloid diseases, such as Alzheimer's and Parkinson's. The preparation of the protein can lead to small amounts of aggregate in the solution, which in turn may lead to irreproducible in vitro results. Using several pre-treatment methods, we have determined that pH cycling and diafiltration of the insuli...

background: the aim of this study was to evaluate the protein spots of excre-tory - secretory products of fasciola hepatica using two dimension electrophoresis method in the presence and absence of triclabendazole drug which can be consid-ered to detect the target protein of the drug. methods: f. hepatica parasites were collected from infected cattle livers, divided in two groups and cultivated...

Amyloid fibrils are associated with a range of human disorders. Understanding the conversion of amyloidogenic proteins from their soluble forms to amyloid fibrils is critical for developing effective therapeutics. Previously we showed that T7 endonuclease I forms amyloid-like fibrils. Here we study the mechanism of the amyloidogenic conversion of T7 endonuclease I. We show that T7 endonuclease ...

Cellular and tissue activities highly depend on environmental pH. Murine lung tissue, when cultured properly in-vitro, is a potent producer of hematopoietic growth factors. We have studied the effect of pH on the production of hematopoietic growth factors and protein synthesis by the murine lung in-vitro. Various concentrations of NaHC03 were used to adjust the pH of the culture medium unde...

in order to study the effects of ph and the ratio of nitrate to ammonium in nutrient solution, on the growth characteristics and on the yield of spinach (spinacia oleracea l.), a factorial experiment was conducted consisting of two factors including: nutrient solution ph in three levels (4.5, 6.5 and 8) and nitrate to ammonium ratio of nutrient solution in five levels (100:0, 75:25, 50:50, 25:7...

An extension of the stoichiometric displacement (SD) model for the ion-exchange adsorption of dilute proteins is developed which accounts for the effects of hydrogen ion Donnan equilibrium on the protein charge. The ability of the new model to fit retention data when the fluid phase pH is near the protein pI and the effects of hydrogen ion Donnan equilibrium are important is examined using four...