Date palm tissue culture technique aimed to production of huge numbers of plants to farmers in order to increasing dates which have high nutritional value. Date palm plantlets from pre-acclimatization stage in vitro were transferred to acclimatize in the greenhouse cultured in peat moss + perlite 2:1 and kept in the plastic covered inside 100% humidity and 28 C0 till new leave or root was grown...

Plantlets under in vitro conditions transferred to ex vivo conditions are exposed to biotic and abiotic stresses. Furthermore, in vitro regenerated plants are typically frail and sometimes difficult to handle subsequently increasing their risk to damage and disease; hence acclimatization of these plantlets is the most important step in tissue culture techniques. An experiment was conducted unde...

Zygotic embryos excised from immature green fruits of the rattan palm, Calamus thwaitesii and cultured for 16 weeks under optimum culture conditions in Murashige and Skoog (MS) medium supplemented with 31.67 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 35.23 μM 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) produced mixed (compact and friable) calli at 70 and 92 % rates. The semi-friable part of ...

The influences of flax lignin and phenolic compounds obtained from spruce bark on the development of Lycopersicon esculentum plantlets were evaluated. Depending on the applied treatment and the concentrations used, the natural aromatic compounds had stimulatory effects on the germination capacity and the plantlets height and leaf area. The influence of lignin on Lycopersicon esculentum was lowe...

A simple method was developed to permit albino plant regeneration from anther culture of Hobigonj Boro (Hbj B) IV and Hbj B VI, two local varieties of aromatic indica rice from Bangladesh. Three crucial factors were identified for the albino shoot primordia to change into green plantlets in culture; components of M10 induction medium, callus size (range 0.2-0.4 cm long) and height of shoot prim...

Genetically modified crops which had been commercial applied extensively majorly are the insect resistance and herbicide tolerance events. In this study, the Bt insecticidal gene Cry1Ab, the glyphosate-tolerant gene EPSPS, and the selection marker gene PMI were combined into a single transferred DNA fragment and introduced into sugarcane by Agrobacterium-mediated transformation. Thirty-three re...

In vitro inoculation of Vitis vinifera L. cv. Chardonnay explants with a plant growth-promoting rhizobacterium, Burkholderia phytofirmans strain PsJN, increased grapevine growth and physiological activity at a low temperature. There was a relationship between endophytic bacterial colonization of the grapevine plantlets and their growth at both ambient (26 degrees C) and low (4 degrees C) temper...

Grapevine (Vitis vinifera L.) is rich in phenylpropanoid compounds, namely flavonoids and stilbenes which, present in most tissues, are described as antioxidants and known to accumulate in response to biotic and abiotic stress. Grapevine is then a choice model for studying the interplay between the phenylpropanoid pathway and nutrient deficiency. Here we report the response to sulfur deficiency...

In order to establish in vitro protocols for raising aseptic cultures of cotyledon segments excised from five-week-old raised seedlings were used as explant material. Among surface sterilants, mercuric chloride 0.1% three minutes provided least rate contamination (24%) and the maximum survival (76%). The highest per cent culture establishment 97.79% was obtained explants on Murashige Skoog (MS)...