Hemorrhagic shock (HS) is associated with high mortality. A severe decrease in blood pressure causes the intestine, a major site of digestive enzymes, to become permeable - possibly releasing those enzymes into the circulation and peritoneal space, where they may in turn activate other enzymes, e.g. matrix metalloproteinases (MMPs). If uncontrolled, these enzymes may result in pathophysiologic ...

background: proteolytic enzymes have an important role in variety of physiological and pathological functions. they have been used in therapeutic and pharmaceutical applications. characterizations of extracellular proteases from various strains of s. marcescens indicate that most strains produce a very similar major metalloprotease. this metalloprotease (serrapeptidase, serrapeptase) is an impo...

background and objectives: psychrotrophic bacteria can produce enzymes at low temperatures; this provides a wide biotechnological potential, and offers numerous economical advantages over the use of mesophilic bacteria. in this study, extracellular protease production by psychrotrophic rheinheimera sp. (km459533) was optimized by the response surface methodology. materials and methods: the cult...

Abstract In this research, the impacts of various enzymes (phospholipase-A 2 (0.3% v/v), lipase (0.03% w/v), and protease (0.5% w/v)) on physico-chemical functional characteristics treated egg white protein (EWP) were determined. The pH, turbidity, colour (L*, a*, b*, ∆E*), gas concentrations in package, relative foaming capacity (RFC), stability analysed during storage at 4 °C. (1,000 ± 60.82)...

The aim of this study was identifying a suitable food grade enzymes to hydrolyze whey protein concentrates (WPCs), to give the highest bioactivity. WPCs from ultrafiltration retentate were adjusted to 35% protein (WPC-35) and hydrolyzed by enzymes, alcalase, α-chymotrypsin, pepsin, protease M, protease S, and trypsin at different hydrolysis times (0, 0.5, 1, 2, 3, 4, and 5 h). These 36 types of...

The production of a protease and a lipase from Bacillus pumilus SG2 on solid-state fermentation using Pongamia pinnata seed cake as substrate was studied. The seed cake was proved to be a promising substrate for the bacterial growth and the enzyme production. The initial pH, incubation time and moisture content were optimized to achieve maximal enzyme production. Maximum protease production was...

The physical, chemical, and immunologic properties of a protease from rat skeletal muscle, proposed to function in the degradation of certain intracellular enzymes, are identical to those of a chymotrypsin-like serine protease isolated from peritoneal mast cells. The results of polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and 8 M urea indicate that the two rat pr...

Halophilic bacteria are a group of microorganisms living in saline environments and some cases need the salinity to survive. Furthermore,these species have potential for interesting promising applications. In fact, this is due not only their ability adapt extreme physico-chemical conditions but also because many them they produce substances industry such as extracellular hydrolytic enzymes.The ...

Protease enzymes are that able to hydrolyze peptide bonds in proteins into simple compounds. The protease enzyme used was derived from fermented sea cucumber digestive organs (HSFI-8). HSFI-8 uses meat tenderizer as well an anticoagulant agent. samples were beef, chicken and tuna whose protein profile analyzed using SDS-PAGE. Three types of soaked at a concentration 30% v/v for 3 h. In addition...