The advent of high-throughput sequencing has allowed genome wide profiling of histone modifications by Chromatin ImmunoPrecipitation (ChIP) followed by sequencing (ChIP-seq). In this assay the histone mark of interest is enriched through a chromatin pull-down assay using an antibody for the mark. Due to imperfect antibodies and other factors, many of the sequenced fragments do not originate fro...

Static circuit gates are the standard circuit devices used to build the major parts of digital circuits. Dynamic gates, such as domino circuits, are only used in certain sections of the circuit where speed is critical. These gates achieve higher speed compared to static gates at the cost of reduced noise margins. We propose a novel gate structure that tries to improved noise margin when compare...

[Purpose] The purpose of this study was to examine the effects of foot placement on the latissmus dorsi and low back muscles activities during pull-down exercise. [Subjects] For this study, we recruited 10 male volunteers. [Methods] The subjects performed pull-down exercises with 4 foot placement conditions. The activities of the latissmus dorsi and L4 paraspinal muscles were measured. [Results...

Cytosolic foreign DNA is detected by pattern recognition receptors and mainly induces type I IFN production. We found that transfection of different types of DNA into various untreated cells induces type III IFN (IFN-λ1) rather than type I IFN, indicating the presence of uncharacterized DNA sensor(s). A pull-down assay using cytosolic proteins identified that Ku70 and Ku80 are the DNA-binding p...

In extension to previously applied techniques like yeast two-hybrid and GST pull-down assays, we successfully established a FACS-based FRET analysis to investigate the interaction of the Mint3 adaptor protein and the small Rab GTPase Rab6A in living mammalian cells. A Mint3 mutant containing only the PTB domain (Mint3Delta6) is able to interact with the constitutively active form of Rab6A. Mint...

A novel high voltage (HV) modulator that offers a short fall time to minimize sputtering effects and allow more precise control of the incident ion fluence in plasma immersion ion implantation is described. The use of 36 insulated-gate bipolar transistors in the 30 kV hard-tube pulser reduces the HV fall time to 3.5 μs, compared to a fall time of 80 μs if a pull-down resister is used. The volta...

Building a usable menu bar, related pull-down menus and submenus or cascaded menus remains an important design activity in the development of interactive applications, especially in the domain of business oriented ones. To provide some assistance to designers who are responsible for achieving this task, a twophased design method for a menu bar and related pull-down menus is presented. Based on ...

In this paper, a new technique for domino circuit is proposed, which has high noise immunity and consume low power without degrading the performance for wide fan-in dynamic gates. The proposed circuit utilizes the double stage domino technique, in which the domino circuit is divided into two stages: standard footed domino includes pull-down network and another standard footed domino includes on...

Multiple endocrine neoplasia type 1 (MEN1) is a syndrome characterized by tumors in multiple endocrine tissues such as the parathyroid glands, the pituitary gland, and the enteropancreatic neuroendocrine tissues. MEN1 is usually caused by mutations in the MEN1 gene that codes for the protein menin. Menin interacts with proteins that regulate transcription, DNA repair and processing, and mainten...