Transmissible plasmids are responsible for the spread of genetic determinants, such as antibiotic resistance or virulence traits, causing a large ecological and epidemiological impact. Transmissible plasmids, either conjugative or mobilizable, have in common the presence of a relaxase gene. Relaxases were previously classified in six protein families according to their phylogeny. Degenerate pri...

Genetic rearrangements within a population of bacteria were analyzed to understand the degree of divergence occurring after experimental evolution. We used 18 replicate populations founded from Ralstonia sp. strain TFD41 that had been propagated for 1,000 generations with 2,4-dichlorophenoxyacetic acid (2,4-D) as the carbon source. Genetic divergence was examined by restriction fragment length ...

BACKGROUND We designed allele-specific primers to amplify genomic DNA of patients with Charcot-Marie-Tooth 1A (CMT1A) and hereditary neuropathy with liability to pressure palsies (HNPP). METHODS Genomic DNA analysis was performed on 40 unrelated CMT1A duplication patients, 25 unrelated HNPP deletion patients, and 50 unaffected control individuals. The CMT1A and HNPP patients had previously be...

The Lactobacillus plantarum and pentosus genotypes existing in industrial-scale cucumber fermentations were defined using rep-PCR-(GTG)5. ability of each genotype to ferment cucumbers under various conditions was evaluated. Rep-PCR-(GTG)5 the technique capable illustrating most intraspecies discrimination compared sequencing housekeeping genes (recA, dnaK, pheS rpoA), MLST RAPD with primers LP1...

There are mainly two categories for a 3D reconstruction from 2D drawings: B-Rep and CSG that both these methods have serious weaknesses despite being useful. B-Rep method which has been older and have wider function range is problematic because of high volume of calculations and vagueness in answers and CSG method has problem in terms of very limited range of volumes and drawings that it can an...

The replicator (rep) of the nopaline-type Ti plasmid pTiC58 is located adjacent to the trb operon of this conjugal element. Previous genetic studies of this region (D. R. Gallie, M. Hagiya, and C. I. Kado, J. Bacteriol. 161:1034-1041, 1985) identified functions involved in partitioning, origin of replication and incompatibility, and copy number control. In this study, we determined the nucleoti...