Elastase LasB, an important extracellular virulence factor, is shown to play an important role in the pathogenicity of Pseudomonas aeruginosa during host infection. However, the role of LasB in the life cycle of P. aeruginosa is not completely understood. This report focuses on the impact of LasB on biofilm formation of P. aeruginosa PAO1. Here, we reported that the lasB deletion mutant (ΔlasB)...

Interfacial properties rhamnolipids from an extract produced by a strain of Pseudomonas aeruginosa were analyzed in this study. The extract of rhamnolipid was characterized by surface tension in different conditions; interfacial tension with different hydrocarbons; critical micelle concentration under different pH and temperatures; particle size and emulsification capacity using laser light pro...

Surfactants are chemical products widely used in our daily life in toothpaste and other personal hygiene and cosmetic products, and in several industries. Biosurfactants are surfactants of biological origin that can be produced by microorganisms and have many advantages, such as low toxicity and high biodegradability, compared to synthetic counterparts. Unfortunately, high production costs limi...

background in recent decades, biological properties of biosurfactants, particularly glycolipids, including antimicrobial, antifungal, and anti-cellular effects have been projected in many research studies. the unique advantages of these compounds in terms of production and environment made them important as antimicrobial agents. objectives this study aims to evaluate probable antibacterial and ...

Nanodiscs are membrane mimetics which may be used as tools for biochemical and biophysical studies of a variety of membrane proteins. These nanoscale structures are composed of a phospholipid bilayer held together by an amphipathic membrane scaffold protein (MSP). In the past, nanodiscs were successfully assembled with membrane scaffold protein 1D1 and 1,2-dipalmitoyl-sn-glycero-3-phosphorylcho...

a pseudomonas aeruginosa mutant derived by random mutagenesis with n-methyl-n¢-nitro-n-nitrosoguanidine, producing high levels of the rhamnolipid biosurfactants was selected on siegmund-wagner (sw) plates. the mutant designated p. aeruginosa ptcc1637 produces rhamnolipids at concentrations 10 time more than parent strain. nmr analysis and surface tension measurement showed that the biosurfactan...

In this paper, we investigated the abilities of five sugar-based synthetic surfactants and biosurfactants from three different families (i.e., alkyl polyglycoside (APG), sophorolipid (SL), rhamnolipid (RL)) to dissolve mobilize non-aqueous phase liquid (NAPL) components, i.e., toluene perchloroethylene (PCE), adsorbed on porous matrices. The objective study was establish a benchmark for selecti...

Background Sinusitis affects significant percentage of population causing considerable long term morbidity. P.aeruginosa is responsible for about 13% of the sinusitis cases. Pseudomonas produces hemolysin, rhamnolipid, a membrane glycolipid, secretory pigments like pyocyanin and 1hydrophenazine which can reduce ciliary beating and mucociliary clearance. Alginate functions as an adhesion, thereb...