the effect of eucalyptus (eucalyptus globulus labill;egeo) and angelica (heracleum persicum desf. ex fischer; hpeo) essential oils was assessed at the levels of 0, 3, 30, 300 or 3000 (µl 30 ml-1) on in vitro fermentation of buffered rumen fluid from 3 castrated male sheep. the fermentation kinetics were estimated after 54 hours incubation. the fermentation kinetic values, in vitro gas productio...

In in vitro ruminal fermentation, micronised wood powder is digested to produce volatile fatty acids, but its fermentability is weak. The aim of the present study was to ascertain a suitable pretreatment of micronised wood powder to enhance volatile fatty acid production in vitro. Rumen fluid taken from beef cattle was anaerobically incubated with micronised wood powder. Initially, effects of p...

The effects of rumen buffer agents on ruminal fermentation parameters and bacterial community composition were determined using in vitro vivo experiments three rumen-cannulated, high-concentrate fed Holstein Friesian dairy cows. Experiment 1 treatments included bentonite, calcium carbonate, oxide, sodium bicarbonate, sesquicarbonate, processed coral, unbuffered samples served as the control. 2 ...

The effect of substituting native grass with jengkol (Archidendron jiringa) by-product on fermentation characteristics, rumen microbial profile, methane production, and hydrogen balance using in vitro method was investigated. Seven treatments (different composition of native grass, jengkol peel, jengkol leaves, and concentrate) with five replications in a block randomized desi...

BACKGROUND The behaviour of perfluoroalkyl acids (PFAAs) in tissues of ruminants has been shown to differ from that of monogastrics (J Agric Food Chem 61(12):2903-2912 doi:10.1021/jf304680j, 2013; J Agric Food Chem 62(28):6861-6870, 2014). This may be a consequence of the complex microbial ecosystem in the rumen. To evaluate this hypothesis, the recovery of PFAAs was studied using the rumen sim...

The primary growth of lucerne (Medicago sativa) was ensiled after treatment with either formic acid alone (4.1 litres/t; silage F) or with formic acid and either formaldehyde (30.5 g/kg crude protein (nitrogen X 6.25; CP); silage FF), glutaraldehyde (44.2 g/kg CP; silage FG) or a mixture of the two aldehydes at approximately half their individual application rates (silage FFG). Compared with fo...

BACKGROUND Understanding the host impact on its symbiotic microbiota is important in redirecting the rumen microbiota and thus improving animal performance. The current study aimed to understand how rumen microbiota were altered and re-established after being emptied and receiving content from donor, thus to understand the impact of such process on rumen microbial fermentation and to explore th...

1. Responses to twice-weekly oral supplementation with 4.0 g methionine were measured with lambs (27 kg) grazing kale (Brassica oleracea) for 10 weeks (Expt 1). In a second experiment with sheep fed on kale at hourly intervals, rumen fractional outflow rates of CrEDTA and ruthenium Tris(1,10 phenanthroline) markers were measured from the rates of decline in their concentrations. Rumen turnover ...

The present study was carried out to evaluate the effect of methanolic extracts of three plants, mehandi (Lawsonia inermis), jaiphal (Myristica fragrans) and green chili (Capsicum annuum) on methanogenesis, rumen fermentation and fermentation kinetic parameters by in vitro gas production techniques. Single dose of each plant extract (1 ml / 30 ml buffered rumen fluid) and two sorghum fodder con...