Abstract Protein engineering has great potential for devising multifunctional recombinant proteins to serve as next-generation protein therapeutics, but it often requires drastic modifications of the parental scaffolds e.g., additional domains at N/C-terminus or replacement a domain by another. A discovery platform system, called RaPID (Random non-standard Peptides Integrated Discovery) enabled...

Milk proteins provide a natural source of bioactive peptides with potential health benefits and applications in the food industry. The release of these peptides from milk proteins is achieved either by hydrolysis using digestive proteases or by lactic acid bacteria fermentation. Peptides, particularly those derived from milk proteins, can exert a wide range of nutritional, functional and biolog...

Introduction: A noninvasive method of detecting exposure of phosphatidylserine (PS) on the external surface of the plasma membrane such as nuclear imaging could assist the diagnosis and therapy of apoptosis related pathologies. The most studied imaging agent for apoptosis is Annexin V so far. Because of limitations of Annexin V other agents have been introduced such as small peptides and molecu...

The aim of this account is to summarize current views on the absorption of the products of protein digestion, amino acids and peptides. Longer reviews of protein absorption (Wiseman, 1968; Matthew, 1969, 1971; Gray & Cooper, 1971) will provide references not given here. Hormonal effects on absorption are reviewed by Levin (1969). Protein digestion is initiated in the stomach by pepsins and cont...

Application of chemotherapy in cancerous children leads to reduction of immune system efficiency. Therefore, these children are prone to various infectious diseases. The excessive use of antibiotics can bring about antibiotic resistant strains. Hence, it is essential to investigate new therapies for this problem. On the other hand, the emergence of resistance against multiple drugs is a major p...

autophagy is a cellular process mediating degradation of bulk cytoplasm, long-lived proteins and entire organelles. in this process, double-membrane vesicles, the autophagosomes, wrap around portions of cytosol and transport them to the lysosome for degradation. several molecules participate in autophagosome nucleation and elongation, including various components of the class iii pi3k complex, ...

Photoaffinity labeling followed by tandem mass spectrometry is an often used strategy to identify protein targets of small-molecule drugs or drug candidates, which, under ideal conditions, enables the identification actual binding site. In case bioactive peptides, however, identifying distinct site hampered because complex fragmentation patterns during spectrometry. We here report development a...

Recently researchers have identified several short, α-helical peptide sequences that are capable of transporting large protein cargo or hydrophilic molecules such as dyes or drugs through the lipid bilayer into cells. These peptides, known as cellpermeable peptides or transduction peptides, have found applications in both basic research and therapeutic purposes. However, peptides are expensive ...

Mass spectrometry (MS) was employed to detect and structurally characterize peptides in two functionally related neurons, named VD1 and RPD2, which form a network involved in the modulation of heartbeat in Lymnaea. Matrix-assisted laser desorption/ionization MS, directly applied to single neurons VD1 and RPD2, showed overlapping yet distinct mass profiles, with a subset of putative peptides spe...