The Hepatitis B Surface antigen ( HBsAg) gene was isolated from an Iranian HBeAg positive carrier by PCR. The gene was cloned in pUC19 for sequencing and pYES2 for expression in Saccharomyces cerevisiae, which pNF1 and pDF3 constructs were made respectively. The sequencing data showed that the isolated HBsAg gene shared more than 90% homology with the ayw subtype. The pDF3 was transferred into ...

The synthesis and transport of the soluble antigen of influenza A virus were traced by immunofluorescent staining with human convalescent sera during abortive and complete infection of hamster BHK21 ceils and mouse L ceils. Times of synthesis in the nucleus, transport to the cytoplasm and extrusion by the cell were the same in complete and in abortive infection. The product of abortive infectio...

Detection of antigen-specific CD4+ T cells is facilitated by the use of fluorescently labeled soluble peptide-major histocompatibility complex (MHC) multimers which mirror the antigen specificity of T-cell receptor recognition. We have used soluble peptide-MHC class II tetramers containing peptides from the protective antigen (PA) of Bacillus anthracis to detect circulating T cells in periphera...

Objective(s): Dendritic cells (DCs) play a critical role in activation of T cell responses. Induction of type1 T helper (Th1) immune response is essential to generate protective immunity against cutaneous leishmaniasis. The intrinsic tendency of liposomes to have interaction with antigen-presenting cells is the main rationale to utilize liposomes as antigen carriers. In the present study, the e...

A complement-fixing antigen specific for scrub typhus occurs in the body fluids and tissues of infected mice, white rats, and cotton rats. The specific serological substance is demonstrable only in those animals which develop a rapidly fatal disease after an incubation period of a few days. Such an experimental infection is induced in mice and rats by the intravenous injection of suspensions of...

Two different precipitating antigens have been extracted from vaccinia virus. One, designated the LS antigen (1), is a protein with two antigenically active groups (2, 3); the other, termed the NP antigen, is a nudeoprotein (4). These two antigens apparently comprise a large proportion of the mass of the virus particle. The soluble complement-fixing antigen of influenza virus is usually extract...