نتایج جستجو برای: species specific primers

تعداد نتایج: 1529553  

A. A. Daee A. Derakhshandeh A. Khodakaram-Tafti, M. Seyedin

Background: Pneumonia due to Mycoplasma infections can cause serious health problems and economic losses in small ruminants industry. Aims: The aim of this study was isolation and identification of Mycoplasmas in sheep naturally infected with pneumonia in Northeastern Iran. Methods: This study used histopathology, culture and ...

2015
Jong-Man Yoon

Genomic DNAs were extracted from the muscle of twenty-one specimens of three eel species collected in Anguilla japonica (AJ), Muraenesox cinereus (MC) and Conger myriaster (CM) from the Yellow Sea, respectively. In the present study, 7 oligonucleotides primers generated 191 specific loci in the AJ species, 226 in the (MC) species and 181 in the CM species, respectively. The primer BION-02 gener...

Journal: :Journal of applied genetics 2002
Ewa Stepniak Maria M Zagalska Marek Switoński

The RAPD-PCR technique was applied to identify genetic markers able to distinguish between four canid species: the arctic fox (Alopex lagopus), red fox (Vulpes vulpes), Chinese raccoon dog (Nyctereutes procyonoides procyonoides) and six breeds of the domestic dog (Canis familiaris). A total of 29 ten-nucleotide arbitrary primers were screened for their potential use in the differentiation of th...

2005
Mun-Ok Kim Gi-Young Kim Byung-Hyouk Nam Cheng-Yun Jin Ki-Won Lee Jae-Min Park Sang-Joon Lee Jae-Dong Lee

Genus Phellinus taxonomically belongs to Aphyllophorales and some species of this genus have been used as a medicinal ingredients and Indian folk medicines. Especially, P. linteus and morphological-related species are well-known medicinal fungi that have various biological activities such as humoral and cell-mediated, anti-mutagenic, and anti-cancer activities. However, little is known about th...

2012
Robert J. Clifford Michael Milillo Jackson Prestwood Reyes Quintero Daniel V. Zurawski Yoon I. Kwak Paige E. Waterman Emil P. Lesho Patrick Mc Gann

Within the paradigm of clinical infectious disease research, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa represent the four most clinically relevant, and hence most extensively studied bacteria. Current culture-based methods for identifying these organisms are slow and cumbersome, and there is increasing need for more rapid and accurate molecular...

Journal: رستنیها 2016

In this study, Dunaliella has been isolated in Jun. 2014 from Hoz-e Soltan and Aran-va-Bidgol salt Lakes (Iran). The isolates have been identified morphologically with the aid of simple light and invert microscope after isolation and purification on their specific medium (Moh202). By using molecular techniques such as polymerase chain reaction (PCR) and amplification of 18S rDNA gene primers, f...

Journal: :Annals of botany 2005
Malay DAS Samik Bhattacharya Amita Pal

BACKGROUND AND AIMS The aim of this study was to develop species-specific molecular markers for Bambusa balcooa and B. tulda to allow for their proper identification, in order to avoid unintentional adulteration that affects the quality and quantity of paper pulp production. METHODS Two putative, species-specific RAPD markers, Bb836 for B. balcooa and Bt609 for B. tulda were generated using a...

2017
S. Sabahi L. Fekrat M. Zakiaghl

Quick and authentic identification of exotic and potentially invasive taxa with capability of causing high economic losses or detriments is essential prerequisite for effective plant quarantine and biological control initiatives. The order Thysanoptera includes several agricultural pest species that, not only because of their minute size but also due to their cryptic behavior, incline to undete...

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