The development of a T7 RNA polymerase (T7 RNAP) expressing cell line i.e. BSR T7/5 cells marks an improvement reverse genetics for the recovery recombinant Newcastle disease virus (rNDV). is developed by transient transfection plasmid encoding RNAP gene rNDV rescue. However, expression decreases gradually over multiple passages and eventually hinders rescue rNDV. To address this issue, lentivi...

Municipal and industrial wastewater treatment plants produce large amounts of sludge. This excess sludge is an inevitable drawback inherent to the waste activated sludge process. Both the reduction of the amount of sludge produced and improving its dewaterability are of paramount importance. Novel pre-treatment processes have been developed in order to improve sludge dewatering, handling and di...

An oil pipeline ruptured in May 1994 and 2 700 tons of crude oil leaked into the São Sebastião Channel, affecting several neighboring areas. A program for the monitoring of hydrocarbons in sediments, using the gas chromatography / flame ionization detector methodology, was being undertaken in the area at the time. The data obtained were compared to those of samples collected after the accident ...


 A pot experiment was carried out to check the impact of different organic manures and inorganic fertilizers on growth yield turnip, at Horticulture Departmental experimental area, Faculty Agriculture, Gomal University, D.I. Khan, during 2012-13. The laid in RCD with seven treatments replicated three times. Treatments contained control (no fertilizer added), FYM @ 25 t/hec, Poultry manure...

To determine the role of protein kinase Cdelta in mouse skin carcinogenesis, we have developed transgenic FVB/N mouse lines expressing in the epidermis an epitope-tagged protein kinase Cdelta (T7-PKCdelta) regulated by the human keratin 14 promoter. The untreated T7-PKCdelta mice displayed excessive dryness in the skin of the tail with a variable penetrance over time. Histologically, the tail s...

The inability of T7 to develop in cells of Escherichia coli containing F(+) or substituted F' episomes is a result of the failure to synthesize late proteins; no in vivo translation of mRNA species synthesized by the T7 RNA polymerase occurs. Further experiments have been performed to measure the amount of late mRNA in T7-infected F'(PIF(+)) cells. (We have designated the property of phage inhi...

Tujuan penelitian ini yaitu untuk mengetahui jumlah lemak yang dihasilkan setiap lapisan atau tumpukan pada suhu ruang dan 60°C serta besar tekanan dibutuhkan pengepresan tiap lapisan/tumpukan biji kakao. Prosedur kerja dilakukan dalam adalah sebagai berikut : (1) Pengupasan Testa Kakao, (2) Pengeringan Pengpresan Nib Kakao dengan perlakuan 3 lapis nib kakao, 5 7 (3) Analisa Kadar Lemak/minyak,...

BACKGROUND Breast conserving therapy (BCT) is an accepted treatment for early-stage breast cancer. This study aimed to measure prospectively acute radiation-related toxicity and to create a comprehensive data base for long-term temporal analyses of 3D conformal adjuvant radiotherapy. The specific aspect of age has been neglected by traditional research. Therefore, the impact of age on acute BCT...