Arsenic activates nuclear factor erythroid 2-related factor 2 (Nrf2) to induce phase II and antioxidative genes. Here we analyzed arsenic-Kelch-like ECH-associated protein 1 (Keap1) cysteine thiol interaction in Nrf2 activation. Arsenic-based Nrf2 activators, fluorescent biarsenical labeling reagent (FlAsH) and phenylarsine oxide (PAO), were used to probe binding of arsenic to Keap1. Strong flu...

Cytochrome P450 (CYP)2C9 and CYP2C19 are important human enzymes that metabolize therapeutic drugs, environmental chemicals, and physiologically important endogenous compounds. Initial studies using primary human hepatocytes showed induction of both the CYP2C9 and CYP2C19 genes by tert-butylhydroquinone (tBHQ). As a pro-oxidant, tBHQ regulates the expression of cytoprotective genes by activatio...

Antiestrogens are thought to exert most of their beneficial effects in breast cancer by antagonizing the actions of estrogen. We report here that antiestrogens also stimulate the expression of quinone reductase (QR) [NAD(P)H:quinone oxidoreductase, EC 1.6.99.2], which may provide protective effects against the toxicity and mutagenicity caused by quinones. QR is up-regulated by low concentration...

The current study was designed to identify the time-dependent gene expression profiles of antioxidant responsive element (ARE)-driven genes induced by tert-butylhydroquinone (tBHQ). A set of simple noise-filtering methods was introduced to evaluate and minimize the variance of microarray datasets. Gene expression induced by tBHQ (10 microM) in IMR-32 human neuroblastoma cells was analyzed by me...

measuring the rate of changes in total polar compounds (tpc) content and polar fractions, the oxidative stability of canola oil during frying process as affected by bene kernel oil (bko, 0.05 and 0.1%) and tert-butylhydroquinone (tbhq, 100ppm) was investigated. it was observed that the tpc content increased linearly with frying time (r2 > 0.98). the canola oil containing the bko (especially 0.1...

This work examines pressurized accelerated oxidation by a RapidOxy tester as an alternative method for determination of biodiesel stability. Sunflower oil-based was synthesized and treated with antioxidants: tert-butylhydroquinone (TBHQ) - synthetic antioxidant known its powerful protective effect, mixture bio-based compounds extracted from vinery waste, VWEeth. The potency TBHQ evaluated at va...