Amyloid fibrils are large aggregates of misfolded proteins, which are often associated with various neurodegenerative diseases such as Alzheimer's, Parkinson's, Huntington's, and vascular dementia. The amount of hydrogen sulfide (H2S) is known to be significantly reduced in the brain tissue of people diagnosed with Alzheimer's disease relative to that of healthy individuals. These findings prom...

Higher house dust levels of PBDE flame retardants (FRs) have been reported in California than other parts of the world, due to the state's furniture flammability standard. However, changing levels of these and other FRs have not been evaluated following the 2004 U.S. phase-out of PentaBDE and OctaBDE. We analyzed dust collected in 16 California homes in 2006 and again in 2011 for 62 FRs and org...

The analysis of the complexes between metal-based chemotherapeutic drugs and proteins in biological samples, such as cisplatin or oxaliplatin, can be a challenge due to metal strong reactivity towards S-donor molecules such as dithiothreitol (DTT) or β-mercaptoethanol (BME), usually employed as reducing agents in electrophoretic separations and proteolytic digestions for LC-MS/MS analysis.•This...

Protein splicing is a self-catalyzed and spontaneous post-translational process in which inteins excise themselves out of precursor proteins while the exteins are ligated together. We report the first discovery of an intramolecular disulfide bond between the two active-site cysteines, Cys1 and Cys+1, in an intein precursor composed of the hyperthermophilic Pyrococcus abyssi PolII intein and e...

The polyelectrolytes poly(L-lysine) (PLL, Mw = 2.60 × 10 Da) and hyaluronic acid (HA, Mw = 1.32 × 10 Da) were purchased from Sigma Aldrich (Saint-Quentin Fallavier, France) and Lifecore Biomedical (Chaska, USA) respectively. Coupling agents as N,N′Dicyclohexylcarbodiimide 99% (DCC), N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride ≥ 98% (EDC), N-Hydroxysuccinimide 98% (NHS), and NHy...

While inward remodeling of small arteries in response to low blood flow, hypertension, and chronic vasoconstriction depends on type 2 transglutaminase (TG2), the mechanisms of action have remained unresolved. We studied the regulation of TG2 activity, its (sub) cellular localization, substrates, and its specific mode of action during small artery inward remodeling. We found that inward remodeli...

The present study evaluated the efficiency of a semi-closed, tubular, horizontal photobioreactor (PBR) to treat mixture irrigation and rural drainage water, focusing in removal different contaminants emerging concern (CECs), evaluating environmental impact resulting effluent. Target CECs included pharmaceuticals, personal care products flame retardants. Of 13 compounds evaluated, 11 were detect...

DNA-based nano-biosensors are emerging scope in the field of biosensors. Many synthetic single stranded functional DNAs have been applied for development of such sensors, recently. Immobilization of DNA oligonucleotides on the surface of gold nanoparticles is a key step in the development of most colorimetric nano-biosensors. The bound DNA is usually thiolated and forms Au-S covalent bond to th...