BACKGROUND Mild solubilization of inclusion bodies has attracted attention in recent days, with an objective to preserve the existing native-like secondary structure of proteins, reduce protein aggregation during refolding and recovering high amount of bioactive proteins from inclusion bodies. RESULTS Here we presented an efficient method for mild solubilization of inclusion bodies by using a...

Cell culture from vials usually includes an open phase performed under laminar flow hoods. Even if disposable and small closed systems are available, at least the first cell culture step after vial thawing, which is a centrifugation, is performed in disposable but open containers. This step is critical as the risk of contamination is high and it also has a direct impact on process timelines. In...

The study was conducted to determine the influence of D-aspartic acid (D-Asp) as antioxidant supplement and thawing rates on ram sperm motility, membrane integrity, abnormality, viability, mitochondria activity, malondialdehyde and antioxidant activities, and total antioxidant capacity after freezing-thawing process. Semen samples from five mature rams (3-4 years old) were diluted with extender...

the use of various slags as by-products of steel industry is well established in civil engineering applications. however, the use of bos slag in the area of soil stabilization has not been fully researched and developed despite having similar chemical composition and mineralogy to that of portland cement. this paper reports on efforts to extend the use of bos slag to soil stabilization by deter...

We use a dynamical systems approach to study thawing quintessence models, using a multiparameter extension of the exponential potential which can approximate the form of typical thawing potentials. We impose observational constraints using a compilation of current data, and forecast the tightening of constraints expected from future dark energy surveys, as well as discussing the relation of our...

The influence of the freeze-thawing process on the size and lamellarity of multilamellar PEG-lipid liposomes prepared from a mixture of egg yolk phosphatidilcholine (EggPC) and distearoyl phosphatidylethanolamine polyethyleneglycol (DSPE-PEG) 2000 was investigated. Trapped volume measurement, quasielastic light scattering (QELS) and freeze-fracture electron microscopy were used to estimate the ...

Dalton et al. (2004) conducted a field trial with Holstein dairy cattle to determine: 1) the effect of simultaneous thawing of multiple 0.5-mL straws of semen and sequence of insemination (1, 2, 3 or 4) on conception rates, 2) whether conception rates achieved following AI by professional AI technicians (PAI) and herdsman-inseminators (HI) differed, and 3) the effect of elapsed time from initia...

BACKGROUND: Freezing is a common way and one of the best methods of seafood preservation for long periods of time; however, the freeze thawing process may influence the quality of food. OBJECTIVES: Oxidation and denaturation of proteins, sublimation and recrystallization of ice crystals can cause changes in the quality of the frozen products. This study was aimed to evaluate the effect of three...

We assessed the damage to onion tissue due to freeze-thawing as the water permeability determined by using PFG-NMR and light microscopy. The water diffusion in fresh onion tissue was restricted due to cellular barriers, and the estimated water permeability was 6.99 x 10(-6)m/s. The water diffusion became considerably less restricted after freeze-thawing; the convergent value for the restricted ...

The aim of the present work was to investigate how colour changes of foal meat can vary after thawing out in relation to the slaughtering age of the horses and to the post-thawing time. Eighteen Italian Heavy Draught Horse (IHDH) foals were used for the trial. They were subdivided in three groups according to their slaughtering age (6, 11 and 18 months). Two different surfaces were investigated...