Angiogenesis is an important component in the development of thyroid goitre. Vascular endothelial growth factor (VEGF) represents a family of specific endothelial cell mitogens involved in normal angiogenesis and in tumour development. The purpose of this study was to determine the distribution of VEGF in thyroid tissues during goitre formation, and to study the actions of VEGF on the regulatio...

ααα-Trifluorothymidine (TFT), an anticancer nucleoside analog, is a potent thymidylate synthase inhibitor. TFT exerts its antitumor activity primarily by inducing DNA fragmentation after incorporation of the triphosphate form of TFT into the DNA. Although an oral combination of TFT and a thymidine phosphorylase inhibitor has been clinically developed, there is little information regarding TFT a...

Under the stress of ultraviolet radiation some cyanobacteria synthesize scytonemin, a protective pigment against DNA photodamage. In addition to photoprotection, scytonemin has been shown have an anti-proliferative effect on various types malignant cells. this study melanoma and spleen cells was assessed both in vitro using tissue cultures vivo mice models. Melanoma were exposed 0.08 10 μM cell...

A long-acting thymidine pellet consisting of 190 mg of cholesterol and 60 mg of thymidine has been developed for the study of thymidine metabolism and reutilization in vivo. Implantation of such a pellet s.c. in adult mice will maintain the blood plasma concentration of thymidine at levels between 40 and 8 X 10(-6) M, which are from 36 to 7 times those of normal mice, for periods up to 48 hr. D...

Cells in culture can condition the medium they grow in so as to inhibit cellular uptake of nucleosides. Some conditioned media specifically block the uptake of cytosine arabinoside and deoxycytidine, but not other deoxyribonucleosides; some also block thymidine uptake; some have no effect. Detransformation of cells by sodium butyrate can alter their medium-conditioning properties. The inhibitio...

Nucleoside-derived drugs play an important role in the treatment of cancer. Here, we present the synthesis and characterization of an intriguing series of N3 conjugated Re(CO)(3) thymidine complexes. The complexes were characterized by NMR spectroscopy and mass spectrometry and their cytotoxicity was assessed against A549 cells. A similar dependence on the spacer length and the toxicity has bee...

The loss of intrinsic neurons is an early event in inflammation of the rat intestine that precedes the growth of intestinal smooth muscle cells (ISMC). To study this relationship, we cocultured ISMC and myenteric plexus neurons from the rat small intestine and examined the effect of scorpion venom, a selective neurotoxin, on ISMC growth. By 5 days after neuronal ablation, ISMC number increased ...

Administration of cortisol to mice bearing the cortisol sensitive or -resistant lines of lymphosarcoma P1 798 resulted in a marked inhibition of incorporation of labeled deoxy nucleoside precursors into tumor DNA only in the sensitive tumor. This effect is one of the earliest biochemical changes see n in lymphosarcoma P1798 following glucocorticoid treatment. It is dependent on both time and do...

Bodmer, Walter F. (Stanford University School of Medicine, Palo Alto, Calif.), and Susan Grether. Uptake and incorporation of thymine, thymidine, uracil, uridine, and 5-fluorouracil into the nucleic acids of Bacillus subtilis. J. Bacteriol. 89:1011-1014. 1965.-From 55 to 95% of uracil, uridine, or 5-fluorouracil (FU) added to the culture medium is incorporated into the acid-insoluble fraction o...