We have developed an activator/repressor expression system for budding yeast in which tetracyclines control in opposite ways the ability of tetR-based activator and repressor molecules to bind tetO promoters. This combination allows tight expression of tetO- driven genes, both in a direct (tetracycline-repressible) and reverse (tetracycline-inducible) dual system. Ssn6 and Tup1, that are compon...

Circuit design for control of metabolism A transcriptional mechanism in yeast that allows cells to respond changes nutrient concentrations works very much like a household light-dimmer switch. That is, the system separately controls whether gene expression is “on” or “off” and extent expression. The galactose-responsive pathway activated when need switch from metabolizing glucose galactose. Ric...

Drought is a major abiotic stress limiting agricultural crops production worldwide. In our study, we isolated novel C2H2-type zinc finger protein gene ZF2 from chickpea. consisted of 232 amino acids with two QALGGH motifs in Cys2/His2 domain. Transient expression analysis ZF2:GFP fusion showed that was nuclear localized protein. the yeast assay system, full-length did not show transcriptional a...

Abstract Fungal unspecific peroxygenases (UPOs) represent an enzyme class catalysing versatile oxyfunctionalisation reactions on a broad substrate scope. They are occurring as secreted, glycosylated proteins bearing haem-thiolate active site and rely hydrogen peroxide the oxygen source. However, their heterologous production in fast-growing organism suitable for high throughput screening has on...

Berberine was loaded in yeast cells of Saccharomyces cerevisiaeas a novel pharmaceutical carrier to improve the treatment ofmany diseases. The yeast-encapsulated active materialsshowedhigh stability and bioavailability due to the enhanced solubility and sustained releasing. In this study, different characteristics of prepared berberine loaded yeast cells (loading capacity, release kinetic order...

Aquaporins 1 (AQP1) and 2 (AQP2) were expressed in the yeast secretory mutant sec6-4. The mutant accumulates post-Golgi, plasma membrane-targeted vesicles and may be used to produce large quantities of membrane proteins. AQP1 or AQP2 were inducibly expressed in yeast and were localized within isolated sec6-4 vesicles by immunoblot analysis. Secretory vesicles containing AQP1 and AQP2 exhibited ...

interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (hcl). the aim of the present study is to apply yeast strain for secreting human ifnα2b fol...

Abstract Engineering nitrogen fixation in eukaryotes requires high expression of functional nitrogenase structural proteins, a goal that has not yet been achieved. Here we build knowledge-based library containing 32 nifH sequences from prokaryotes diverse ecological niches and metabolic features combine with rapid screening tobacco to identify superior NifH variants for plant mitochondria expre...

Background: The most important virulence factor which plays a central role in Candida albicans pathogenesis is the ability of this yeast to alternate between unicellular yeast and filamentous hyphal forms. Efg1 protein is thought to be the main positive regulating transcription factor, which is responsible for regulating hyphal-specific gene expression under most conditions. ALS3 is one of the ...