نتایج جستجو برای: β-galactosidase

تعداد نتایج: 182872  

In this study, immobilization of partially purified almond (Amygdalus communis) β-galactosidase on Con A layered calcium alginate-cellulose beads was investigated. Immobilized β-galactosidase retained 72% of theinitial activity after crosslinking by glutaraldehyde. Both soluble and immobilized enzyme exhibited the samepH and temperature optima at pH 5.5 and 50ºC, respectively. Howev...

Journal: :applied food biotechnology 0
saeed abbasalizadeh agriculture biotechnology research institute of iran, northwest and west region, food biotechnology department, tabriz, iran. mohammad amin hejazi agriculture biotechnology research institute of iran, northwest and west region, food biotechnology department, tabriz, iran. mahdi pesaran hajiabbas agriculture biotechnology research institute of iran, northwest and west region, food biotechnology department, tabriz, iran.

the potential of bulk starter fermentation strategy for production of a cost-effective and safe source of β-galactosidase was investigated. three different bulk starter media with different compositions were selected, and an industrial yogurt starter culture strain, l. bulgaricus dsm 20081 was cultivated in these media under ph-controlled conditions (ph=5.6) at 43°c. the media consisted of 1) b...

Journal: :Journal of Biological Chemistry 1972

Journal: :Journal of Investigative Dermatology 2023

Objective:To investigate whether Opsins are associated with UVR induced nevus activation. Method: Human specimens Dispase digestion at 4°C overnight.The epidermis was discard and digested dermis 0.2% type collagenase 37°C for 2-3h to obtained cells.Culture cells medium 254 human melanocyte growth factor.Nevus divided into BRAF wild BRAFV600E mutant by Sanger sequencing.Analysis of SA-β -galacto...

Background: Aging is one of the important factors in the development of age-related diseases. Acrylamide can be produced during carbohydrate-rich foods prepared at high temperatures. Recently, studies showed that acrylamide can induce cellular senescence. On the other hand, Rutin as a natural flavonoid, has a potent antioxidant activity. Objective: This study aims to evaluate the ptotective ef...

Journal: :Journal of Applied Phycology 2023

Abstract This study investigated the mechanism of lactose assimilation in Nannochloropsis oceanica for dairy-wastewater bioremediation and co-production valuable feed/food ingredients a circular dairy system (β-galactosidase omega-3 polyunsaturated fatty acids). Mixotrophic cultivation was found to be mandatory N. , with biomass production mixotrophic cultures reaching fourfold increase over th...

2016
Mizuki Tomizawa Kohei Tsumaki Masaki Sone

β-Galactosidase encoded by the Escherichia coli lacZ gene, is widely used as a reporter molecule in molecular biology in a wide variety of animals. β-Galactosidase retains its enzymatic activity in cells or tissues even after fixation and can degrade X-Gal, a frequently used colormetric substrate, producing a blue color. Therefore, it can be used for the activity staining of fixed tissues. Howe...

2005
IVA TROJANOVÁ

TROJANOVÁ I., RADA V. (2005): Enzymatic activity in fermented milk products containing bifidobacteria. Czech J. Food Sci., 23: 224–229. Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus and Bifidobacterium animalis strains were tested for α-galactosidase, β-galactosidase and α-glucosidase activities. Commercially available yoghurts with bifidobacteria were also tested. Whi...

2014
Judy Chang Shannon Coleman Young Eun Lee Tegan Schellenberg-Beaver

The purpose of this study was to determine if the osmoregulatory response in Escherichia coli BW25993 varied depending on the solute used to cause osmotic stress, as measured by β-galactosidase enzyme activity. β-galactosidase enzyme activity was measured in E. coli BW25993 after osmotic shock with NaCl or sucrose for 2 hours. β-galactosidase activity was normalized against number of colony for...

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