Eight lepidopteran cell lines were established recently and their susceptibility to different insect viruses was studied. Two Spodoptera litura cell lines from the larval and pupal ovaries, were found highly susceptible to S. litura nuclear polyhedrosis virus (SLNPV, 5-6 x 10(6) NPV/ml). The Helicoverpa armigera cell line from the embryonic tissue was highly susceptible to H. armigera NPV (HaNP...

In this study we developed the transposon-mediated shuttle vector 'Hanpvid', which composed of HaNPV (Heliothis armigera nuclear polyhedrosis virus) genomic DNA and a transposon cassette from Bacmid of Bac-to-Bac system. Hanpvid replicates in E. coli in the same way as Bacmid and retains infective function in cotton bollworm cells (Hz-AM1). Using Hanpvid we constructed a recombinant virus, whic...

Mutualistic associations between symbiotic bacteria and their hosts are common within insect systems. However, viruses are often considered as pathogens even though some have been reported to be beneficial to their hosts. Herein, we report a novel densovirus, Helicoverpa armigera densovirus-1 (HaDNV-1) that appears to be beneficial to its host. HaDNV-1 was found to be widespread in wild populat...

Field trials were conducted on the efficacy of different control options against Helicoverpa armigera tomato. We evaluated and compared pest control, safety to natural enemies, crop yield economics application for various treatments including: a mycoinsecticide based Beauveria bassiana; baculovirus, H. nucleopolyhedrovirus (HaNPV); plant extract from Dodonaea viscosa; insecticide chlorantranili...

The investigations on “Grain yield and incremental cost benefit ratio (ICBR) biorational insecticides against Helicoverpa armigera in chickpea” were carried out at the Student’s Instructional Farm, C. S. Azad University of Agriculture Technology, Kanpur during Rabi season 2021-22 2022-23 with three replications eight treatments insecticides, armigera. like HaNPV, Nimbecidine, Bacillus thuringie...

A study of histopathological changes due to the infection of Helicoverpa armigera Nuclear Polyhedrosis Virus (HANPV) in the third instar larvae of H. armigera at 24, 48, 72 and 96 hour interval was carried out in the laboratory. The histopathalogical sequential changes in the post infected H. armigera revealed the presence of infected area in the midgut epithelium at 72 hpi (hour post infection...

The experiment was conducted at the research plot of Department Agricultural Entomology Central Research Field, Sam Higginbottom University Agriculture Technology and Sciences, Prayagraj, India, during Rabi season 2022. treatments selected for this were Emamectin benzoate, Beauveria bassiana, Spinosad, Heterorhabditis indica, Metarhizium anisopliae, HaNPV, Bacillus thuringiensis control to obse...