A "microgram assay" is described in which solid-phase enzyme-linked immunosorbent assay is used for the determination of specific rubella immunoglobulin G (IgG) antibody levels in micrograms per milliliter of serum. The quantitation was based on a standard curve obtained by using a reference serum, for which the specific IgG content was assayed by immunochemical purification. IgG was first puri...

in this study canthaxanthin was produced by dietzia natronolimnaea hs1 and used for incorporation in niosomes after extraction. effect of rotational speed of rotary vacuum evaporator flask (100 and 150 rpm), canthaxanthin concentration (0, 1 and 2 microgram/ milliliter), concentration of poly ethylene glycol 400 (0, 0.1 and 0.2 %), surfactant type (tween 60 and 80, span 60 and 80) and surfactan...

Although cycloheximide is extremely inhibitory to protein synthesis in vivo in higher plants, the reported insensitivity of some plant ribosomes suggests that it may not invariably act at the ribosomal level. This suggestion is reinforced by results obtained with red beet storage tissue disks, the respiration of which is stimulated by cycloheximide at 1 microgram per milliliter. Inorganic ion u...

A procedure for purifying 2,2', 4,4',5,5'-hexabromobiphenyl (HBB) from FireMaster FF-1 in gram amounts by crystallization is presented. Following purification, the structural assignment of HBB was made by using proton and carbon-13 nuclear magnetic resonance (NMR) spectroscopy, elemental analysis, and mass spectroscopy (MS). The growth of C3H/10T 1/2 cells treated with 5, 37, 75, and 150 microg...

Submit your manuscript here: http://www.dovepress.com/clinical-ophthalmology-journal Clinical Ophthalmology is an international, peer-reviewed journal covering all subspecialties within ophthalmology. Key topics include: Optometry; Visual science; Pharmacology and drug therapy in eye diseases; Basic Sciences; Primary and Secondary eye care; Patient Safety and Quality of Care Improvements. This ...

In vitro comparison on dermatophytes showed, on a microgram-per-milliliter basis, that tolciclate was more active than clotrimazole and miconazole in inhibiting the growth of dermatophytes in Sabouraud agar with and without 10% horse serum.

Cathespin D from the retinal pigment epithelium of bovine eyes was purified about 25-fold from a crude extract of retinal pigment epithelium by acid treatment, ammonium sulfate fractionation, and Sephadex G-200 column chromatography. The purified enzyme hydrolyzed bovine serum albumin optimally at pH values close to 4.0. Exposure of the enzyme to 60 degrees C. for 2 minutes resulted in 50 per c...

Ion and oxygen uptake were studied on aging bean stem slices. Oxygen uptake was high immediately after slicing, decreased to a minimum at 100 minutes, and then increased again. Ion uptake per unit of O(2) uptake data suggested that metabolic energy was utilized almost exclusively for sodium transport in fresh tissue but was diverted to potassium transport as the slices aged. Oxygen and ion upta...