نتایج جستجو برای: 5-enoylpyruvyl shikimate-3-phosphate synthase

تعداد نتایج: 2612807  

Ali Hatef Salmanian, Amir Mousavi, Haleh Hashemi Sohi Mahyat Jafari Mehrnoosh Fathi Roudsari

Presence of antibiotic resistance markers has always been considered as one of the main safety concerns in transgenic plants and their derived products. Elimination of antibiotic selectable markers from transgenics is a major hurdle for finding efficient and safe candidates. Herbicide tolerance genes might be attractive alternatives. In this study, a variant form of the 5-enoylpyruvyl shikimate...

Journal: :iranian journal of biotechnology 2009
mehrnoosh fathi roudsari ali hatef salmanian amir mousavi haleh hashemi sohi mahyat jafari

presence of antibiotic resistance markers has always been considered as one of the main safety concerns in transgenic plants and their derived products. elimination of antibiotic selectable markers from transgenics is a major hurdle for finding efficient and safe candidates. herbicide tolerance genes might be attractive alternatives. in this study, a variant form of the 5-enoylpyruvyl shikimate...

Journal: :Archives of biochemistry and biophysics 2006
Júlio C Borges José H Pereira Igor B Vasconcelos Giovanni C dos Santos Johnny R Olivieri Carlos H I Ramos Mário S Palma Luiz A Basso Diógenes S Santos Walter F de Azevedo

The 5-enolpyruvylshikimate-3-phosphate synthase catalyses the sixth step of the shikimate pathway that is responsible for synthesizing aromatic compounds and is absent in mammals, which makes it a potential target for drugs development against microbial diseases. Here, we report the phosphate binding effects at the structure of the 5-enolpyruvylshikimate-3-phosphate synthase from Mycobacterium ...

Journal: :Plant physiology 1988
J E Ream H C Steinrücken C A Porter J A Sikorski

5-Enolpyruvylshikimate-3-phosphate (EPSP) synthase (3-phospho-shikimate 1-carboxyvinyltransferase; EC 2.5.1.19) was purified 1300-fold from etiolated shoots of Sorghum bicolor (L.) Moench. Native polyacrylamide gel electrophoresis revealed three barely separated protein bands staining positive for EPSP synthase activity. The native molecular weight was determined to be 51,000. Enzyme activity w...

Journal: :The Biochemical journal 1993
Q K Huynh

Photo-oxidation of Escherichia coli 5-enolpyruvoylshikimate-3-phosphate synthase, a target for the non-selective herbicide glyphosate (N-phosphonomethylglycine), in the presence of pyridoxal 5'-phosphate resulted in irreversible inactivation of the enzyme. The inactivation followed pseudo-first-order and saturation kinetics with a Kinact. of 50 microM. The inactivation is specifically prevented...

Journal: :Plant physiology 1984
J L Rubin C G Gaines R A Jensen

Treatment of isogenic suspension-cultured cells of Nicotiana silvestris Speg. et Comes with glyphosate (N-[phosphonomethyl]glycine) led to elevated levels of intracellular shikimate (364-fold increase by 1.0 millimolar glyphosate). In the presence of glyphosate, it is likely that most molecules of shikimate originate from the action of 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthase...

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