We examined cell cycle–dependent changes in the proteome of human cells by systematically measuring protein dynamics in individual living cells. We used time-lapse microscopy to measure the dynamics of a random subset of 20 nuclear proteins, each tagged with yellow fluorescent protein (YFP) at its endogenous chromosomal location. We synchronized the cells in silico by aligning protein dynamics ...

Ageing is a complex multifactorial process, reflecting the progression of all degenerative pathways within an organism. Due to the increase of life expectancy, in recent years, there is a pressing need to identify early-life events and risk factors that determine health outcomes in later life. So far, genetic variation only explains ∼20–25% of the variability of human survival to age 80+. This ...

Aortic arch (AA) is a prolongation of aorta ascendens and forms a part of mediastenum superius. It begins right above the joint of sternum and the second rib at the posterior right half of manubrium sterni. Presently, aorta has been pointed out not only to perform the duty of a canal but in fact has been shown to have a significant role in regulating left ventricular function and myocardial per...

CONTEXT AND OBJECTIVE Prader-Willi syndrome (PWS) is characterized by early-onset hyperphagia and increased circulating levels of the orexigenic Acylated Ghrelin (AG) hormone with a relative deficit of Unacylated Ghrelin (UAG). AZP-531, a first-in-class UAG analog, was shown to inhibit the orexigenic effect of AG in animals, to improve glycemic control and decrease body weight in humans. We aim...

In order to assess the level-2 data retrieval accuracy of selected trace gases (O3, NO2, H2O) from SCIAMACHY and MIPAS, which are part of the atmospheric instrumentation on the recently launched ENVISAT satellite, operational and some scientific products are validated by comparison with other space borne instruments (GOME, HALOE, SAGE II, POAM III). First results of these satellite intercompari...

There are some reports which indicate that several triazole derivatives have inotropic activity; however, the cellular site and mechanism of action at cardiovascular level is very confusing. In order, to have a clear understanding of these phenomena, a triazole derivative was synthesized to evaluate its biological activity on left ventricular pressure and characterize the molecular mechanism. T...

Abstract Background The learning opportunities for global health professionals have expanded rapidly in recent years. diverse array of learners and wide range course quality underscore the need an improved vetting process to better match with appropriate opportunities. Methods We developed a framework assess overall by determining performance across four defined domains Relevance, Engagement, A...

BACKGROUND Most current treatments for chronic immune thrombocytopenic purpura (ITP) act by decreasing platelet destruction. In a phase 1-2 study, we administered a thrombopoiesis-stimulating protein, AMG 531, to patients with ITP. METHODS In phase 1, 24 patients who had received at least one treatment for ITP were assigned to escalating-dose cohorts of 4 patients each and given two identical...