Several viruses can cause devastating diseases in domestic apple (Malus x domestica Brokh.). Major viruses affecting apple production in New York are apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV). These viruses are common and generally latent; in other words, they do not cause acute symptoms on most commercial cultivars. However, when...

Deep sequencing has generated 52 contigs derived from five viruses; Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV), Apple green crinkle associated virus (AGCaV), and Apricot latent virus (ApLV) were identified from eight apple samples showing small leaves and/or growth retardation. Nucleotide (nt) sequence identity of the assembled con...

Background and Aims: In spite of a long tradition of fruit-tree growing in all provinces of Iran, information on pear (Pyrus communis) tree viruses is scant. In order to identify of the nature of the virus like symptoms and decline in pear trees, we carried out a survey for the occurrence of four important viruses. Materials and Methods: Leaf samples of pear showing virus like symptoms wer...

Selected problems related to non-uniformity of plant virus sequence variants present in infected plants and imperfectness of current diagnostic methods are illustrated and discussed. A failure of some primers to react (efficiently) with atypical isolates/strains of Apple chlorotic leaf spot virus (ACLSV) and Plum pox virus (PPV) is demonstrated. On the other hand, problematic positive reactions...