dog rose (rosa canina) has been using as a rootstock for ornamental roses also it is one of the medicinal plants. it can be propagated under in vitro conditions. after removing chilling requirement of buds, axillary wood buds of dog rose were cut in three nodal positions (lower, middle and terminal) on the stem and then explants after decontamination, cultured on murashige and skoog medium (ms ...

Dog rose (Rosa canina) has been using as a rootstock for ornamental roses also it is one of the medicinal plants. It can be propagated under in vitro conditions. After removing chilling requirement of buds, axillary wood buds of dog rose were cut in three nodal positions (lower, middle and terminal) on the stem and then explants after decontamination, cultured on Murashige and Skoog medium (MS ...

Nodal explants bearing a single axillary bud, from three cultivars of cultivated grape, viz., `Thompson seedless', `Sonaka' and `Tas-e-Ganesh', were used to initiate shoot cultures on G16 medium containing adenine sulphate, monobasic sodium phosphate, BAP and NAA. Each shoot, developed from an axillary bud, produced a tuft of multiple shoots on a medium containing BAP, calcium pantothenate, mon...

We established sterile clones of a medicinal plant dragonhead Dracocephalum charkeviczii Prob. (Lamiaceae). used shoots the plants from natural habitat, notably leaf nodes with axillary bud, as explants, sterilized explants in 0.1 % Diocide for 4 minutes and cultivated them on agarized nutrient half-strength MS medium vitamins, 2 sucrose, 0.4 agar, 1 mg/l indolylbutyric acid (IBA), or 0.5 1.0 6...

In vitro budinduction and shoot regeneration of Lantana camara L. was investigated using axillary nodal explants. Multiple shoot buds were induced on Murashige and Skoog (MS) media supplemented with 0, 2, 4 and 8 mg/l of 6-benzylaminopurine (BAP) or thidiazuron (TDZ) for proliferation and 0, 0.25 and 0.5 mg/l indole-3 butyric acid (IBA) for rooting. After 4 weeks, a maximum number of shoot buds...

Cyperus scariosus R.Br is a valuable multipurpose medicinal herb, which belongs to family Cyperaceae. It possesses astringent, anti-inflammatory, diaphoretic, diuretic, cordial and stomach ache properties. An attempt has been made to study in vitro regeneration, floral bud induction, multiple shooting and multiple rooting has been achieved via healthy, moderate axillary buds as explants. The ax...

A protocol for the micropropagation of Eucalyptus polybractea R.T. Baker (blue mallee) using axillary bud proliferation from lignotuber-derived explants is described. Three different ages of plants were used as explant sources: glasshouse-grown seedlings, field-grown saplings, and coppice of field-grown mature lignotubers. Explants from each source initiated successfully and no significant diff...

A reliable method is described for rapid proliferation of axillary shoots from nodal segment of mature field-grown aromatic and medicinal plant Vitex negundo through in vitro culture. Of different concentrations of N6-benzyladenine (BA) evaluated as supplements to Murahige and Skoog (MS) medium, BA at 1mg/l was effective in enhancing bud break and shoot regeneration. Response of explants was al...