Aiming to develop a DNA marker specific for Bacillus anthracis and able to discriminate this species from Bacillus cereus, Bacillus thuringiensis, and Bacillus mycoides, we applied the randomly amplified polymorphic DNA (RAPD) fingerprinting technique to a collection of 101 strains of the genus Bacillus, including 61 strains of the B. cereus group. An 838-bp RAPD marker (SG-850) specific for B....

Food poisoning laboratories identify Bacillus cereus using routine methods that may not differentiate all Bacillus cereus group species. We recharacterized Bacillus food-poisoning strains from 39 outbreaks and identified B. cereus in 23 outbreaks, B. thuringiensis in 4, B. mycoides in 1, and mixed strains of Bacillus in 11 outbreaks.

background and objectives: research on xylanase has markedly increased due to its potential applications in pulping and bleaching processes using cellulose free preparations, textile processes, the enzymatic saccharification of lignocellulosic materials and waste treatment. the present study was aimed at isolation and characterization of xylan degrading strain of bacillus cereus from soil for p...

Genome features of the Bacillus cereus group genomes (representative strains of Bacillus cereus, Bacillus anthracis and Bacillus thuringiensis sub spp. israelensis) were analyzed and compared with the Bacillus subtilis genome. A core set of 1381 protein families among the four Bacillus genomes, with an additional set of 933 families common to the B. cereus group, was identified. Differences in ...

Fifty nine samples of raw milk, 60 samples of pasteurized milk, 24 cream, 39 butter, 27 curd, 19 peda, 21 paneer and 20 skim milk powder samples were analyzed for the presence of different Bacillus species and a total of 182 isolates were obtained. The isolates were identified as Bacillus subtilis (51), Bacillus megaterium (39), Bacillus cereus (28), Bacillus licheniformis (32), Bacillus coagul...

The identification of Bacillus anthracis involves its differentiation from other aerobic sporeformers, particularly Bacillus cereus. Smith et al. (1952), in a study of aerobic sporeforming bacteria, concluded that B. anthracis is a pathogenic variety of B. cereus. They also stated that strains of B. anthracis which had lost their virulence could not be differentiated from B. cereus. This appare...

Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis are members of the Bacillus cereus group of bacteria, demonstrating widely different phenotypes and pathological effects. B. anthracis causes the acute fatal disease anthrax and is a potential biological weapon due to its high toxicity. B. thuringiensis produces intracellular protein crystals toxic to a wide number of insect larvae...

In order to determine if variations in rRNA sequence could be used for discrimination of the members of the Bacillus cereus group, we analyzed 183 16S rRNA and 74 23S rRNA sequences for all species in the B. cereus group. We also analyzed 30 gyrB sequences for B. cereus group strains with published 16S rRNA sequences. Our findings indicated that the three most common species of the B. cereus gr...

background drug resistance of microorganisms to antimicrobial agents is on the rise. therefore, an alternative route to overcome drug resistance of various microorganisms is needed. objectives this study was conducted to assess antibacterial activity of silver nanoparticles (agnps) produced by satureja hortensis extract against bacillus cereus isolated from soil of sistan plain (zabol, southeas...