the interaction of hgh with some metal ions ( ) at 27°c in nac1 solution, 50 mm was studied using isothermal titration calorimetry. there is a set of three identical and non-interacting binding sites for binding of all these metal ions, expect . the intrinsic association equilibrium constants () are not very different for  and , and also their molar enthalpies of binding (kj/mol for  and  kj/mo...

The interaction of hGH with some metal ions ( ) at 27°C in NaC1 solution, 50 mM was studied using Isothermal titration calorimetry. There is a set of three identical and non-interacting binding sites for binding of all these metal ions, expect . The intrinsic association equilibrium constants () are not very different for  and , and also their molar enthalpies of binding (KJ/mol for  and  KJ/mo...

isotherms of the binding of the anthracyciine antibiotic, adriamycin (adriblastin), to dna histone complexes was studied by means of spectroscopic analysis. the results indicated that: (a) binding of adriamycin to histones reduced the interaction of histones with dna, (b) binding of the drug to dna did not change the binding affinity of histone to dna and, (c) in the explored binding range of r

the object of this cross sectional study was to determine the hcv subtype 3a envelope protein binding affinity with human leukocyte antigen. envelope 1 (e1) protein is one of the structural proteins responsible for entering the cells through the receptors. the binding affinity of e1 protein epitopes to the selected human leukocyte antigen (hla) class i alleles was investigated using the compute...

Human serum albumin (HSA) is the most abundant protein in the blood plasma. Molecular dynamics simulations of subdomain IIA of HSA and its complex with salicylic acid (SAL) were performed to investigate structural changes induced by the ligand binding. To estimate the binding affinity of SAL molecule to subdomains IB and IIA in HSA protein, binding free energies were calculated using the Molecu...

human serum albumin (hsa) is the most abundant protein in the blood plasma. drug binding to hsa is crucial to study the absorption, distribution, metabolism, efficiency and bioavailability of drug molecules. in this study, isothermal titration calorimetry and molecular dynamics simulation of hsa and its complex with indometacin (im) were performed to investigate thermodynamics parameters and th...

Background: The affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as ELISA. Affinity of most IgG specific MAbs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...

Wnts are the major ligands responsible for activating Wnt signaling pathway through binding to Frizzled proteins (Fzd) as the receptors. Among these ligands, Wnt2 plays the main role in the tumorigenesis of several human cancers especially colorectal cancer (CRC). Therefore, it can be considered as a potential drug target.The aim of this study was to identify potential drug candidates ...

Wnts are the major ligands responsible for activating Wnt signaling pathway through binding to Frizzled proteins (Fzd) as the receptors. Among these ligands, Wnt2 plays the main role in the tumorigenesis of several human cancers especially colorectal cancer (CRC). Therefore, it can be considered as a potential drug target.The aim of this study was to identify potential drug candidates ...