the development of culture media able to mimic the preovulatory stage of follicular environment and support nuclear and cytoplasmic maturation of oocyte is important for in-vitro fertilization (ivf) programs. it seems that the best culture media for embryonic development and cell cleavage is the natural composition which surrounds the oocyte which has been used occasionally in human ivf program...

Background: One of the main problems in ART is low pregnancy rates. The factors involved in successful pregnancy include; 1. mother related factors such as the status of endometrial receptivity and hormonal status of the mother. 2. embryo related factors such as; number of transferred embryo, embryo scores including: number and size of the blastomeres, and their fragmentation. The aim of the pr...

introduction: the aim of this study was to investigate the growth and development of mouse two cell embryo on human uterine polarized monolayers. material and methodes: human endometrial tissue was obtained from patients who had undergone total hysterectomy. then, epithelial cells were isolated by collagenase i 0.25% and cultured on either ecm gel coated millipore filter insert (polarized) or p...

background:   objective: the objective of this study was to evaluate the effect of rabbit antiserum to es cells on mouse preimplantation embryo development and chimera production.   materials and methods: mouse 4-cell embryos were matured in vitro at 37.5 o c, in humidified 5% co 2 atmosphere for 12-36 h. the embryos were cultured in ksom medium with or without antiserum for 12-36 h. the ratios...

introduction: to improve the development of embryo in vitro several attempts have been made, including the application of leukemia inhibitory factor (lif). this factor plays an important role in embryo development and has been shown to enhance in vitro development rate in sheep and mice. there is controversy regarding the effect of lif on the different stages of embryo development. the aim of t...

Background The aim of this study was to establish a cell-free sequential culture system that can support high levels of in vitro embryo development and blastocyst formation from bovine zygotes. To this end, this investigation was carried out to evaluate the effects of glucose, serum and EDTA on bovine zygote in vitro development. MaterialsAndMethods Bovine presumptive zygotes were derived from ...

Both cleavage-stage and blastocyst-stage embryo transfer policieshave advantages and drawbacks.Potential advantages of blastocyst culture and transfer in human IVF include: i. synchronization of the embryo with the female tract leading to increasedimplantation rates; thereby, reducing the need for multipleembryo transfers, ii. assessment of viability of an embryobefore transfer. This can be ach...

Cell Suspension Culture, Somatic Embryo Genetic and Synthetic Seed Production in Different Cultivars of Potato

Recently, we demonstrated chromosome instability (CIN) in human cleavage stage embryogenesis following in vitro fertilization (IVF). CIN not necessarily undermines normal human development (i.e. when remaining normal diploid blastomeres develop the embryo proper), however it can spark a spectrum of conditions, including loss of conception, genetic disease and genetic variation development. To s...