The ability to respond rapidly to changes in temperature is critical for insects and other ectotherms living in variable environments. In a physiological process termed rapid cold-hardening (RCH), exposure to nonlethal low temperature allows many insects to significantly increase their cold tolerance in a matter of minutes to hours. Additionally, there are rapid changes in gene expression and c...

BACKGROUND Although cold shock responses and the roles of cold shock proteins in microorganisms containing multiple cold shock protein genes have been well characterized, related studies on bacteria possessing a single cold shock protein gene have not been reported. Thermoanaerobacter tengcongensis MB4, a thermophile harboring only one known cold shock protein gene (TtescpC), can survive from 5...

In this study we investigated the effect of heat shock (42-45°C for 5, 10, 15, 20, 25, 30 minutes) cold shock (4, 0, -5, -10 and -20°C for 2h) on cell wall Microstructure of LAB. Cell wall Changes and micro -damage behavior of LAB under the impact of heat and cold shock was studied by optical microscope and Transmission Electron Microscope (TEM). Treatment of LAB with heat and cold shock had si...

F OUR trials were conducted with boar spermatozoa to determine the effects of dilution rate, seminal plasma content and extender composition on susceptibility to cold shock and the development of resistance to cold during 30 C incubation. Acrosome morphology and sperm motility were used to assess damage by cold shock. The susceptibility of boar spermatozoa to cold shock increased as the dilutio...

Background: Gastric ulcer is among the most serious stomach disorder universally. Several effective drugs are employed in the management of this disease, although there have been adverse effects in some cases. The aim of this study was to investigate the effect of nortriptyline to protect against gastric lesions, induced by indomethacin or cold-stress in rats. Methods: Gastric lesions were ind...

experiment was conducted to evaluate the effect of different levels of egg yolk for coating and storing spermatozoa. ejaculates were collected from four rams. in each session, second ejaculates (n=4) were collected in a tube containing 1 ml coating buffers which were prepared by 10, 15 or 20% egg yolk plus tris-fructose. samples were pooled, centrifuged and the supernatant removed in the labora...

Vibrio parahaemolyticus was subjected to cold shock treatment at 20 or 15 degrees C for 2 or 4 h. The effect of cold shock on the survival of V. parahaemolyticus subjected to subsequent low temperature (5 and -18 degrees C) and other adverse conditions (47 degrees C, 6 ppm crystal violet, 1000 ppm H(2)O(2), 25 mM acetic acid and 25 mM lactic acid) was investigated. Regardless of the cold shock ...

The expression of bacterial cold-shock proteins (CSPs) is highly induced in response to cold shock, and some CSPs are essential for cells to resume growth at low temperature. Bordetella bronchiseptica encodes five CSPs (named CspA to CspE) with significant amino acid homology to CspA of Escherichia coli. In contrast to E. coli, the insertional knock-out of a single csp gene (cspB) strongly affe...