Addition of dihydro-N-demethylstreptomycin (DH-NDMS)or N-demethylstreptomycin (NDMS)in a concentration of lOmg/ml to a growing culture of S. griseus blocked the de novo biosynthesis of streptomycin (SM). A simultaneous addition of 14CH3-L-methionine, however, led to specific N-methylation of the added compounds, thus giving the authentic antibiotics dihydrostreptomycin (DHS) and SM. By using th...

Several families of diuretic hormones exist in insects, one of which is the calcitonin-like diuretic hormone (CT/DH) family. CT/DH mediates its effects by binding to family B G-protein coupled receptors (GPCRs). Here we isolate and functionally characterize two R. prolixusCT/DH receptor paralogs (Rhopr-CT/DH-R1 and Rhopr-CT/DH-R2) using a novel heterologous assay utilizing a modified human embr...

Insect diuretic hormones are crucial for control of water balance. We isolated from the cockroach Diploptera punctata two diuretic hormones (DH), Dippu-DH(31) and Dippu-DH(46), which increase cAMP production and fluid secretion in Malpighian tubules of several insect species. Dippu-DH(31) and -DH(46) contain 31 and 46 amino acids, respectively. Dippu-DH(46) belongs to the corticotropin-releasin...