نتایج جستجو برای: DNA Hybridization

تعداد نتایج: 543321  

نزهت, زهرا , هدایتی, مهدی ,

In situ hybridization (ISH) is a method that uses labeled complementary single strand DNA or RNA to localize specific DNA or RNA sequences in an intact cell or in a fixed tissue section. The main steps of ISH consist of: probe selection, tissue or sample preparation, pre-hybridization treatment, hybridization and washing, detection and control procedure. Probe selection is one of the important ...

Journal: :applied biotechnology reports 0
mohammad ebrahim minaei department of biology, imam hossein university, tehran, iran mojtaba saadati department of biology, imam hossein university, tehran, iran mostafa najafi department of chemistry, imam hossein university, tehran, iran hossein honari department of biology, imam hossein university, tehran, iran

in this study, we explained a nanobiosensor for dna sequence detection, featuring sequence specificity, cost efficiency, speed, and ease of use. without the need for labels or indicators, it may be ideal for the detection of biological agents. this review describes recent advances in electrochemical impedance spectroscopy (eis) with an emphasis on using nanoparticles and nanotechnology. a power...

Journal: :iranian journal of biotechnology 2008
amir lakzian

many researchers consider the use of different probes for hybridization assays as suitable for studying the genetic diversity of nitrogen fixing bacteria. in this study for asessing genetic diversity among bradyrhizobium japonicum isolates, two different probes (suca and topa) chosen from the chromosomal genome of bradyrhizobium strain usda 110 were designed, evaluated by dnaman software and im...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد علوم دارویی - دانشکده داروسازی 1394

حذف ناخالصی های سلول میزبان، مرحله بحرانی در تولید فرآورده های بیولوژیک است. یکی از ناخالصی هایی که باید در مرحله خالص سازی پاکسازی شود باقی مانده dnaای است که از سلول میزبان باقی میماند. یکی از پرمصرف ترین رده های سلولی برای تولید این نوع از فرآورده ها سلول های تخمدان همستر چینی(cho) است که ممکن است مقداری از dna این سلول ها در فرآورده باقی مانده و باعث بروز مشکلات در مصرف کننده شود.علاوه بر ...

Many researchers consider the use of different probes for hybridization assays as suitable for studying the genetic diversity of nitrogen fixing bacteria. In this study for asessing genetic diversity among Bradyrhizobium japonicum isolates, two different probes (sucA and topA) chosen from the chromosomal genome of Bradyrhizobium strain USDA 110 were designed, evaluated by DNAMAN software and im...

Journal: :iranian journal of public health 0
yongzhi lun 1. liaoning provincial university key laboratory of biophysics, college of medicine, dalian university dalian, china. chongbo xu 1. liaoning provincial university key laboratory of biophysics, college of medicine, dalian university dalian, china. qing chi 1. liaoning provincial university key laboratory of biophysics, college of medicine, dalian university dalian, china. xuelei wang 1. liaoning provincial university key laboratory of biophysics, college of medicine, dalian university dalian, china. wen sui 1. liaoning provincial university key laboratory of biophysics, college of medicine, dalian university dalian, china. sujuan jiang 2. department of gynecology and obstetrics, affiliated zhongshan hospital of dalian university , dalian, china.

hepatitis b virus (hbv) dna polymerase transactivated protein 1 (hbvdnaptp1) is a novel protein transactivated by hbv dna polymerase, screened by suppression subtractive hybridization technique (genbank accession no: ay450389). the biological function of hbvdnaptp1 was investigated in this study.we constructed a vector pcdna3.1 (-)/myc-his a-hbvdnaptp1 and used it to transfect acute monocytic l...

Journal: :Proceedings of the National Academy of Sciences 2008

استوری, کریستوفر, حاجیا, مسعود,

Twenty to thirty percent of causative agents of the respiratory tract infections are still unknown, that can be related to the lack of suitable laboratory procedures. Some of these organisms may cause progressive infection such as encephalitis and cardiac complications. To recognize the M. pneumoniae , PCR-hybridization technique was used and a specific internal probe was produced for this...

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