Bioinformatics — the application of computer technology to the management of biological information — is essential to deciphering the genetic code of life. Novel approaches to genome sequencing, such as microarray technology, high-performance supercomputing and computational simulations in high-throughput DNA analysis have led to an explosion of genomic data available. Accurate genomic assembly...

Expressed Sequence Tag (EST) sequencing is a highly efficient technique that samples expressed genes required for most cellular functions. While this is a well-studied problem and many software tools have been developed, large-scale EST clustering has previously been pursued through incremental approaches, a pipeline of programs and manual efforts to achieve a modest degree of parallelism. Here...

BACKGROUND AND AIMS Banana (Musa acuminata) is a crop contributing to global food security. Many varieties lack resistance to biotic stresses, due to sterility and narrow genetic background. The objective of this study was to develop an expressed sequence tag (EST) database of transcripts expressed during compatible and incompatible banana-Mycosphaerella fijiensis (Mf) interactions. Black leaf ...

Background: Recombinant insulin glargine, a long-acting analogue of insulin, is expressed as proinsulin in host cell and after purification and refolding steps cleaved to active insulin by enzymatic digestion using trypsin and carboxypeptidase B. Since the proinsulin's B and C chains have several internal arginine and lysine residues, a number of impurities are generated following treatment wit...